Blood protein adsorption onto a polymeric biomaterial of polyethylene glycol and poly[(2-hydroxyethyl methacrylate)-co-acrylonitrile] and evaluation of in vitro blood compatibility

A semi-interpenetrating polymer network (semi-IPN) of polyethylene glycol (PEG) and crosslinked poly[(2-hydroxyethyl methacrylate)-co-acrylonitrile] was prepared and adsorption of bovine serum albumin (BSA) on the IPN surfaces was investigated. The dynamic nature of the adsorption process was studied, and the effects of various experimental factors such as pH and ionic strength on the adsorption isotherms of BSA were investigated. Various kinetic parameters, such as the adsorption coefficient, rate constant for adsorption and penetration rate constants, were calculated. For assessment of in vitro blood compatibility of the IPN surfaces, water sorption, blood clot formation tests and percent hemolysis measurements were performed. (C) 2004 Society of Chemical Industry.