Analysis of vegetable oil transesterification products by gel permeation chromatography

被引：39

作者：

Darnoko, D ^{[1
]}

Cheryan, M ^{[1
]}

Perkins, EG ^{[1
]}

机构：

[1] Univ Illinois, Dept Food Sci & Human Nutr, Agr Bioproc Lab, Urbana, IL 61801 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2000年 / 23卷 / 15期

关键词：

D O I：

10.1081/JLC-100100491

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for simultaneous analysis of transesterification reaction products - triglycerides, diglycerides, monoglycerides, methyl esters, and glycerol - was developed using gel permeation chromatography. Two Phenogel columns are coupled in series to a refractive index detector, and tetrahydrofuran is used as the mobile phase. Sample preparation involves only dilution and neutralization. The column was calibrated with standard solutions of the compounds. Reproducibility of the method was good: analysis of palm oil transesterification products at different levels of conversion showed a relative standard deviation of 0.27% - 3.87%.

引用

页码：2327 / 2335

页数：9

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