Microsatellite markers for diverse Salix species

被引：50

作者：

Barker, JHA

Pahlich, A

Trybush, S

Edwards, KJ

Karp, A

机构：

[1] Univ Bristol, Dept Agr Sci, IACR, Long Ashton Res Stn, Bristol BS41 9AF, Avon, England

[2] Univ Munich, Inst Biochem, D-81377 Munich, Germany

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 01期

关键词：

microsatellites; polymorphism; polyploidy; Salix; willow;

D O I：

10.1046/j.1471-8286.2003.00332.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Forty-six microsatellites were isolated from an enriched library of Salix butjatica and tested on 20 individuals (of nine species/hybrids) from the National Willows Collection (IACR-Long Ashton Research Station, UK). Twenty-nine were monomorphic, gave multilocus or unscorable patterns, or were duplicates. The remaining 17 microsatellites gave 2-22 alleles/ locus. Three microsatellites successfully cross-amplified in 31 additional Salix species. A further six were tested on panels comprising 6-25 individuals from the 31 species. Cross-amplification was successful in all cases. These results suggest that the microsatellites isolated here should prove useful for population studies in a wide range of Salix species.

引用

页码：4 / 6

页数：3