A FAFLP system for the improved identification of plant-pathogenic and plant-associated species of the genus Pantoea

被引:20
作者
Brady, Carrie [1 ]
Venter, Stephanus
Cleenwerck, Ilse
Vancanneyt, Marc
Swings, Jean
Coutinho, Teresa
机构
[1] Univ Pretoria, FABI, Dept Microbiol & Plant Pathol, ZA-0002 Pretoria, South Africa
[2] Univ Ghent, BCCM, LMG, Bacteria Collect, B-9000 Ghent, Belgium
基金
新加坡国家研究基金会;
关键词
Pantoea; FAFLP; identification;
D O I
10.1016/j.syapm.2007.01.005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The majority of Pantoea species are either plant-pathogenic or plant-associated and cause a wide variety of symptoms on a range of hosts. Identification of Pantoea species is difficult due to minor differences in phenotypic characteristics between them and related Enterobacteriaceae. Fluorescent amplified fragment length polymorphism (FAFLP) analysis was investigated for use as a rapid, molecular-based identification technique to the species level of the genus Pantoea. Following analysis of the band patterns generated by FAFLP, seven distinct clusters were observed, one for each validly published species of the genus. FAFLP has proven to be a rapid, reproducible identification technique for all species of the genus Pantoea. (C) 2007 Elsevier GmbH. All rights reserved.
引用
收藏
页码:413 / 417
页数:5
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