Structures of DNA Polymerases Caught Processing Size-Augmented Nucleotide Probes

被引：20

作者：

Betz, Karin ^{[1
]}

Streckenbach, Frank

Schnur, Andreas

Exner, Thomas

Welte, Wolfram

Diederichs, Kay

Marx, Andreas

机构：

[1] Univ Konstanz, Konstanz Res Sch Chem Biol, Dept Chem, D-78457 Constance, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 30期

关键词：

DNA; DNA polymerase; DNA replication; nucleotides; structure elucidation; ACTIVE-SITE CONSTRAINTS; CRYSTAL-STRUCTURE; REVERSE-TRANSCRIPTASE; REPLICATION FIDELITY; STERIC CONSTRAINTS; TERNARY COMPLEXES; LARGE FRAGMENT; ERROR-PRONE; BETA; MECHANISMS;

D O I：

10.1002/anie.200905724

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The right fit: Synthetic nucleotide analogues are widely used to investigate the mechanisms that govern DNA polymerase selectivity - processes that are crucial for the survival of every living organism. The first crystal structures of size-augmented 4′-methylated and 4′-ethylated thymidine triphosphates (TTPs) in complex with a DNA polymerase have been elucidated (picture: superposition of three DNA polymerase structures in complex with TTPs). (Figure Presented). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5181 / 5184

页数：4

共 51 条

[1] Mismatch-induced conformational distortions in polymerase support an induced-fit mechanism for fidelity [J].

Arora, K ;

Beard, WA ;

Wilson, SH ;

Schlick, T .

BIOCHEMISTRY, 2005, 44 (40) :13328-13341

[2] THE FIDELITY OF TAQ POLYMERASE CATALYZING PCR IS IMPROVED BY AN N-TERMINAL DELETION [J].

BARNES, WM .

GENE, 1992, 112 (01) :29-35

[3] Mechanisms of DNA Polymerases [J].

Berdis, Anthony J. .

CHEMICAL REVIEWS, 2009, 109 (07) :2862-2879

[4] Exploring the effects of active site constraints on HIV-1 reverse transcriptase DNA polymerase fidelity [J].

Cramer, J ;

Strerath, M ;

Marx, A ;

Restle, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (46) :43593-43598

[5] Synthesis and Biological Properties of Cylindramide Derivatives: Evidence for Calcium-Dependent Cytotoxicity of Tetramic Acid Lactams [J].

Cramer, Nicolai ;

Helbig, Sarah ;

Baro, Angelika ;

Laschat, Sabine ;

Diestel, Randi ;

Sasse, Florenz ;

Mathieu, Daniel ;

Richter, Christian ;

Kummerloewe, Grit ;

Luy, Burkhard ;

Schwalbe, Harald .

CHEMBIOCHEM, 2008, 9 (15) :2474-2486

[6] Opposed steric constraints in human DNA polymerase β and E. coli DNA polymerase I [J].

Di Pasquale, Francesca ;

Fischer, Daniela ;

Grohmann, Dina ;

Restle, Tobias ;

Geyer, Armin ;

Marx, Andreas .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (32) :10748-10757

[7] Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution [J].

Doublié, S ;

Tabor, S ;

Long, AM ;

Richardson, CC ;

Ellenberger, T .

NATURE, 1998, 391 (6664) :251-258

[8]

ECHOLS H, 1991, ANNU REV BIOCHEM, V60, P477, DOI 10.1146/annurev.biochem.60.1.477

[9] Computer simulation of the chemical catalysis of DNA polymerases:: Discriminating between alternative nucleotide insertion mechanisms for T7 DNA polymerase [J].

Florián, J ;

Goodman, MF ;

Warshel, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (27) :8163-8177

[10] Computer simulations of protein functions:: Searching for the molecular origin of the replication fidelity of DNA polymerases [J].

Florián, J ;

Goodman, MF ;

Warshel, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (19) :6819-6824

← 1 2 3 4 5 6 →