Delivery of antisense oligonucleotides to neuroblastoma cells

被引：10

作者：

Skalko-Basnet, N ^{[1
]}

Tohda, M ^{[1
]}

Watanabe, H ^{[1
]}

机构：

[1] Toyama Med & Pharmaceut Univ, Inst Nat Med, Dept Pharmacol, Toyama, Japan

来源：

NEUROREPORT | 2000年 / 11卷 / 14期

关键词：

antisense oligonucleotides; NG; 108-15; cells; pH-sensitive liposomes;

D O I：

10.1097/00001756-200009280-00016

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

pH-Sensitive liposomes composed of dioleoylphosphatidylethanolamine and cholesterol hemisuccinate (3:2 mol/mol) were applied in delivery of antisense oligodeoxynucleotides (asODN) into NG 108-15 neuroblastoma and glioma cells. Fluorescently labelled asODN were entrapped in liposomes by a modified freeze-thawing method (20% encapsulation efficiency). The uptake of asODN (free or entrapped in liposomes) by NG 108-15 cells was monitored by fluorescence-activated cell sorting and confocal microscopy. Delivery of asODN was significantly improved when antisense were entrapped in liposomes as compared to free (nonliposomal) asODN. The uptake was dose-dependent and optimum was achieved after 2 h incubation. NeuroReport 11:3117-3121 (C) 2000 Lippincott Williams & Wilkins.

引用

页码：3117 / 3121

页数：5

共 15 条

[1] PHOTODYNAMIC THERAPY OF HUMAN BLADDER-CARCINOMA CELLS IN-VITRO WITH PH-SENSITIVE LIPOSOMES AS CARRIERS FOR 9-ACETOXY-TETRA-N-PROPYLPORPHYCENE [J].

AICHER, A ;

MILLER, K ;

REICH, E ;

HAUTMANN, R .

UROLOGICAL RESEARCH, 1994, 22 (01) :25-32

[2] Biological barriers to cellular delivery of lipid-based DNA carriers [J].

Bally, MB ;

Harvie, P ;

Wong, FMP ;

Kong, S ;

Wasan, EK ;

Reimer, DL .

ADVANCED DRUG DELIVERY REVIEWS, 1999, 38 (03) :291-315

[3] EFFICIENCY OF CYTOPLASMIC DELIVERY BY PH-SENSITIVE LIPOSOMES TO CELLS IN CULTURE [J].

CHU, CJ ;

DIJKSTRA, J ;

LAI, MZ ;

HONG, K ;

SZOKA, FC .

PHARMACEUTICAL RESEARCH, 1990, 7 (08) :824-834

[4]

Couvreur Patrick, 1997, Journal of Liposome Research, V7, P1, DOI 10.3109/08982109709035478

[5] Delivery of antisense oligonucleotides by means of pH-sensitive liposomes [J].

DeOliveira, MC ;

Fattal, E ;

Ropert, C ;

Malvy, C ;

Couvreur, P .

JOURNAL OF CONTROLLED RELEASE, 1997, 48 (2-3) :179-184

[6]

Lai J, 1997, J PHARMACOL EXP THER, V281, P589

[7]

LASIC DD, 1997, LIPOSOMES GENE DELIV

[8] Synthetic DNA delivery systems [J].

Luo, D ;

Saltzman, WM .

NATURE BIOTECHNOLOGY, 2000, 18 (01) :33-37

[9] Optimization of liposome mediated transfection of a neuronal cell line [J].

McQuillin, A ;

Murray, KD ;

Etheridge, CJ ;

Stewart, L ;

Cooper, RG ;

Brett, PM ;

Miller, AD ;

Gurling, HMD .

NEUROREPORT, 1997, 8 (06) :1481-1484

[10] Apoptosis of human neuroblastoma cells induced by liposome-capsulated fenretinide [J].

Pagnan, G ;

Caridi, G ;

Montaldo, PG ;

Bado, M ;

Chiesa, V ;

Allen, TM ;

Ponzoni, M .

JOURNAL OF LIPOSOME RESEARCH, 1998, 8 (03) :401-423

← 1 2 →