Mediated amperometric immunosensing using single walled carbon nanotube forests

被引:63
作者
O'Connor, M [1 ]
Kim, SN
Killard, AJ
Forster, RJ
Smyth, MR
Papadimitrakopoulos, F
Rusling, JF
机构
[1] Dublin City Univ, Natl Ctr Sensor Res, Dublin 9, Ireland
[2] Univ Connecticut, Inst Mat Sci, Polymer Program, Nanomat Optoelect Lab, Storrs, CT 06269 USA
[3] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA
[4] Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06032 USA
关键词
D O I
10.1039/b412805b
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A prototype amperometric immunosensor was evaluated based on the adsorption of antibodies onto perpendicularly oriented assemblies of single wall carbon nanotubes called SWNT forests. The forests were self-assembled from oxidatively shortened SWNTs onto Nafion/iron oxide coated pyrolytic graphite electrodes. The nanotube forests were characterized using atomic force microscopy and resonance Raman spectroscopy. Anti-biotin antibody strongly adsorbed to the SWNT forests. In the presence of a soluble mediator, the detection limit for horseradish peroxidase (HRP) labeled biotin was 2.5 pmol ml(-1) (2.5 nM). Unlabelled biotin was detected in a competitive approach with a detection limit of 16 nmol ml(-1) (16 muM) and a relative standard deviation of 12%. The immunosensor showed low non-specific adsorption of biotin-HRP (approx. 0.1%) when blocked with bovine serum albumin. This immunosensing approach using high surface area, patternable, conductive SWNT assemblies may eventually prove useful for nano-biosensing arrays.
引用
收藏
页码:1176 / 1180
页数:5
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