Triptolide inhibits transcription factor NF-kappaB and induces apoptosis of multiple myeloma cells

被引：86

作者：

Lou, YJ ^{[1
]}

Jie, J ^{[1
]}

Wang, YG ^{[1
]}

机构：

[1] Zhejiang Univ, Affiliated Hosp 1, Inst Hematol, Dept Hematol, Hangzhou 310003, Zhejiang, Peoples R China

来源：

LEUKEMIA RESEARCH | 2005年 / 29卷 / 01期

关键词：

triptolide; multiple myeloma; NF-kappaB; apoptosis; caspase; 3; PARP;

D O I：

10.1016/j.leukres.2004.05.014

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Triptolide has been reported to be effective in the treatment of auto-immune diseases. This study investigates the cytotoxic function of triptolide on multiple myeloma (MM) cells. We found that triptolide inhibited the proliferation of both RPM18226 and U266 cells in a dose-dependent manner (10-80 ng/mL). Triptolide induced apoptosis in MM cells through activation of the cystein protease caspase 8, 9 and 3, and subsequent cleavage of the DNA repair enzyme poly (ADP-ribose) polymerase. Apoptosis was confirmed with cell-cycle analysis and annexin V staining. Moreover, triptolide down-regulated nuclear factor (NF)-kappaB activity in MM cell lines. In addition, triptolide also induced chemosensitivity to doxorubicin and suppressed cell proliferation of fresh MM cells. Therefore, triptolide appears to be a potent inducer of apoptosis in myeloma cells, and might have some benefit in the treatment of myeloma patients. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：99 / 105

页数：7

共 41 条

[1] A prospective, randomized trial of autologous bone marrow transplantation and chemotherapy in multiple myeloma [J].