Glutamate-evoked redox state alterations are involved in tissue transglutaminase upregulation in primary astrocyte cultures

被引：58

作者：

Campisi, A

Caccamo, D

Volti, GL

Currò, M

Parisi, G

Avola, R

Vanella, A

Ientile, R ^{[1
]}

机构：

[1] Univ Messina, Dept Biochem Physiol & Nutr Sci, Policlin Univ, I-98125 Messina, Italy

[2] Catania Univ, Dept Biol Chem Med Chem & Mol Biol, I-95100 Catania, Italy

[3] Catania Univ, Biochem Sect, Dept Chem Sci, I-95100 Catania, Italy

来源：

FEBS LETTERS | 2004年 / 578卷 / 1-2期

关键词：

glutamate; ROS production; primary astrocyte culture; tissue transglutaminase; glutathione; antioxidant;

D O I：

10.1016/j.febslet.2004.10.074

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to evaluate the involvement of oxidative stress in glutamate-evoked transglutaminase (TGase) upregulation in astrocyte cultures (14 DIV). A 24 h exposure to glutamate caused a dose-dependent depletion of glutathione intracellular content and increased the ROS production in cell cultures. These effects were receptor-mediated, as demonstrated by inhibition with GYKI 52466. The pre-incubation with glutathione ethyl ester or cysteamine recovered oxidative status and was effective in significantly reducing glutamate-increased tissue TGase. These data suggest that tissue TGase upregulation may be part of a biochemical response to oxidative stress induced by a prolonged exposure of astrocyte cultures to glutamate. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：80 / 84

页数：5

共 35 条

[1] Mitochondria and cell death - Mechanistic aspects and methodological issues [J].