Were inefficient mitochondrial haplogroups selected during migrations of modern humans? A test using modular kinetic analysis of coupling in mitochondria from cybrid cell lines

被引：55

作者：

Amo, Taku ^{[1
]}

Brand, Martin D. ^{[1
]}

机构：

[1] MRC, Dunn Human Nutr Unit, Cambridge CB2 2XY, England

来源：

BIOCHEMICAL JOURNAL | 2007年 / 404卷 / 02期

基金：

英国医学研究理事会;

关键词：

coupling efficiency; cybrid; human evolution; mitochondrial DNA (mtDNA); modular kinetic analysis; oxidative phosphorylation;

D O I：

10.1042/BJ20061609

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce a general test of the bioenergetic importance of mtDNA (mitochondrial DNA) variants: modular kinetic analysis of oxidative phosphorylation in mitochondria from cybrid cells with constant nuclear DNA but different mtDNA. We have applied this test to the hypothesis [Ruiz-Pesini, Mishmar, Brandon, Procaccio and Wallace (2004) Science 303, 223-226] that particular mtDNA haplogroups (specific combinations of polymorphisms) that cause lowered coupling efficiency, leading to generation of less ATP and more heat, were positively selected during radiations of modern humans into colder climates. Contrary to the predictions of this hypothesis, mitochondria from Arctic haplogroups had similar or even greater coupling efficiency than mitochondria from tropical haplogroups.

引用

页码：345 / 351

页数：7

共 30 条

[1] Unique presence of a manganese catalase in a hyperthermophilic archaeon, Pyrobaculum calidifontis VA1 [J].