High level production, characterization and construct optimization of the ionotropic glutamate receptor ligand binding core

被引：4

作者：

Chen, GQ ^{[1
]}

Gouaux, E ^{[1
]}

机构：

[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

来源：

TETRAHEDRON | 2000年 / 56卷 / 48期

基金：

美国国家科学基金会;

关键词：

glutamate receptor; ligand binding; Escherichia coli; protein production; protein engineering; preparative folding; X-ray crystallography;

D O I：

10.1016/S0040-4020(00)00825-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Multimilligram quantities of active and pure GluR2-S1S2, the recombinant ligand binding core of the AMPA-sensitive GluR2 receptor, were produced by preparative folding of the solubilized inclusion bodies expressed in 11 of Escherichia coli cell culture. The biochemical properties and biological activities of folded protein were characterized and the protein construct was optimized for three-dimensional structural studies. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：9409 / 9419

页数：11

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