Phospholipase Cγ2 is essential in the functions of B cell and several Fc receptors

被引:369
作者
Wang, DM
Feng, J
Wen, RR
Marine, JC
Sangster, MY
Parganas, E
Hoffmeyer, A
Jackson, CW
Cleveland, JL
Murray, PJ
Ihle, JN
机构
[1] St Jude Childrens Res Hosp, Howard Hughes Med Inst, Memphis, TN 38105 USA
[2] St Jude Childrens Res Hosp, Dept Biochem, Memphis, TN 38105 USA
[3] St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA
[4] St Jude Childrens Res Hosp, Div Expt Hematol, Memphis, TN 38105 USA
[5] St Jude Childrens Res Hosp, Dept Infect Dis, Memphis, TN 38105 USA
[6] Univ Tennessee, Sch Med, Dept Biochem, Memphis, TN 38063 USA
关键词
D O I
10.1016/S1074-7613(00)00005-4
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Many receptors activate phospholipase C gamma 1 or -gamma 2. To assess the role of PLC gamma 2, we derived enzyme-deficient mice. The mice are viable but have decreased mature B cells, a block in pro-B cell differentiation, and B1 B cell deficiency. IgM receptor-induced Ca2+ flux and proliferation to B cell mitogens are absent. IgM, IgG2a, and IgG3 levels are reduced, and T cell-independent antibody production is absent. The similarity to Btk- or Blnk-deficient mice demonstrates that PLC gamma 2 is downstream in Btk/Blnk signaling. FcR gamma signaling is also defective, resulting in a loss of collagen-induced platelet aggregation, mast cell Fc epsilon R function, and NK cell Fc gamma RIII and 2B4 function. The results define a signal transduction pathway broadly utilized by immunoglobulin superfamily receptors.
引用
收藏
页码:25 / 35
页数:11
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