A sequential injection method for the determination of piroxicam in pharmaceutical formulations using europium sensitized fluorescence

被引:37
作者
Al-Kindy, SMZ [1 ]
Al-Wishahi, A [1 ]
Suliman, FEO [1 ]
机构
[1] Sultan Qaboos Univ, Coll Sci, Dept Chem, Al Khoud 123, Oman
关键词
sensitized fluorescence; europium; piroxicam; sequential injection;
D O I
10.1016/j.talanta.2004.04.014
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple, selective and sensitive luminescence method for the assay of piroxicam (PX) in pharmaceutical formulation is developed. The method is based on the luminescence sensitization of europium (Eu3+) by complexation with PX. The signal for PX-EU is monitored at lambda(ex) = 358 nm and lambda(em) = 615 nm. Optimum conditions for the formation of the complex in methanol were 0.01 M TRIS buffer and 0.2 mM of Eu3+ which allows the determination of 100-2000 ppb of pX in batch method and 100-1000 ppb with limit of detection (LOD) = 23.0 ppb using sequential injection analysis (SIA). The relative standard deviations of the method range between 2 and 3% indicating excellent reproducibility of the method. The proposed method was successfully applied for he assay of PX in pharmaceutical formulations (Feldene capsules and tablets). Average recoveries of 101.0 +/- 0.3 and 98.8 +/- 2.7% were obtained for capsules in methanol using batch and sequential injection (SI) methods, respectively. (C) 2004 Elsevier B.V. All rights reserved.
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页码:1343 / 1350
页数:8
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