Trans-kingdom conjugation between Agrobacterium tumefaciens and Saccharomyces cerevisiae, a bacterium and a yeast

被引：19

作者：

Sawasaki, Y ^{[1
]}

Inomata, K ^{[1
]}

Yoshida, K ^{[1
]}

机构：

[1] HIROSHIMA UNIV,FAC SCI,DEPT BIOL SCI,LAB MOLEC BIOL & BIOINFORMAT,HIGASHIHIROSHIMA 739,JAPAN

来源：

PLANT AND CELL PHYSIOLOGY | 1996年 / 37卷 / 01期

关键词：

Agrobacterium tumefaciens; conjugative plasmids; Saccharomyces cerevisiae; Ti plasmid; trans-kingdom conjugation;

D O I：

10.1093/oxfordjournals.pcp.a028906

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

For conjugation between prokaryotic Agrobacterium tumefaciens and eukaryotic Saccharomyces cerevisiae, we constructed two novel conjugative plasmids. A. tumefaciens transmitted the plasmids to S. cerevisiae with the aid of tra genes on a helper plasmid. The transmitted plasmids retained their original structure and function in transconjugant yeasts. The presence of Ti plasmid barely affected the trans-kingdom conjugation.

引用

页码：103 / 106

页数：4

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