Discrimination between different types of human adeno-associated viruses in clinical samples by PCR

被引：38

作者：

Tobiasch, E ^{[1
]}

Burguete, T ^{[1
]}

Klein-Bauernschmitt, P ^{[1
]}

Heilbronn, R ^{[1
]}

Schlehofer, JR ^{[1
]}

机构：

[1] Deutsch Krebsforschungszentrum, D-69120 Heidelberg, Germany

来源：

JOURNAL OF VIROLOGICAL METHODS | 1998年 / 71卷 / 01期

关键词：

adeno-associated virus; AAV serotypes; PCR; virus detection;

D O I：

10.1016/S0166-0934(97)00198-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Persistent infection of human tissues with the helper virus-dependent parvovirus: adeno-associated virus (AAV) was detected by polymerase chain reaction (PCR) using primer pairs detecting AAV types 2, 3 or 5. In order to develop PCR protocols which discriminate between the different serotypes of AAV, the DNA of AAV-5 was sequenced partially and compared with the published sequences of AAV-2 and -3. Type specific oligonucleotides and specific probes which allow the distinction between human AAV types by PCR are described. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：17 / 25

页数：9

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