Effects of iron enrichment on phytoplankton in the Southern Ocean during late summer: active fluorescence and flow cytometric analyses

被引:90
作者
Olson, RJ [1 ]
Sosik, HM [1 ]
Chekalyuk, AM [1 ]
Shalapyonok, A [1 ]
机构
[1] Woods Hole Oceanog Inst, Dept Biol, Woods Hole, MA 02543 USA
基金
美国国家航空航天局; 美国国家科学基金会;
关键词
D O I
10.1016/S0967-0645(00)00064-3
中图分类号
P7 [海洋学];
学科分类号
0707 ;
摘要
Eight shipboard iron-enrichment experiments were carried out during the late summers of 1997 and 1998 in the Ross Sea and the Polar Front, respectively, as part of the US JGOFS Southern Ocean program. Using active fluorescence techniques (pump-during-probe flow cytometry/microfluorometry and fast repetition rate fluorometry) and flow cytometry, we examined responses of phytoplankton to iron enrichment over time scales of days. Results of both individual cell and bulk water measurements suggest that physiological iron limitation was widespread in the Ross Sea gyre in the late summer, but that in the region just south of the Polar Front other factors were limiting phytoplankton growth. In the five experiments in which responses to enrichment occurred, all the phytoplankton groups we examined, with the exception of cryptophytes, responded to iron enrichment by increasing normalized variable fluorescence (F-v/F-m) over several days. Normalized variable fluorescence of cryptophyte cells was typically higher than that of other cells and often near the maximum observed. Significant correlations were observed between ambient iron concentrations and normalized variable fluorescence at the beginning of each experiment, and also between ambient iron and the response of normalized variable fluorescence to enrichment. These relationships, which have not been previously documented, support the use of ambient active fluorescence measurements to predict iron-limiting conditions without conducting incubations. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:3181 / 3200
页数:20
相关论文
共 34 条
  • [1] Confirmation of iron limitation of phytoplankton photosynthesis in the equatorial Pacific Ocean
    Behrenfeld, MJ
    Bale, AJ
    Kolber, ZS
    Aiken, J
    Falkowski, PG
    [J]. NATURE, 1996, 383 (6600) : 508 - 511
  • [2] BOYD P, 1999, US JGOFS NEWSLETTER, V9, P10
  • [3] METAL ENRICHMENT EXPERIMENTS IN THE WEDDELL-SCOTIA SEAS - EFFECTS OF IRON AND MANGANESE ON VARIOUS PLANKTON COMMUNITIES
    BUMA, AGJ
    DEBAAR, HJW
    NOLTING, RF
    VANBENNEKOM, AJ
    [J]. LIMNOLOGY AND OCEANOGRAPHY, 1991, 36 (08) : 1865 - 1878
  • [4] Differential response of equatorial Pacific phytoplankton to iron fertilization
    Cavender-Bares, KK
    Mann, EL
    Chisholm, SW
    Ondrusek, ME
    Bidigare, RR
    [J]. LIMNOLOGY AND OCEANOGRAPHY, 1999, 44 (02) : 237 - 246
  • [5] ON IRON LIMITATION OF THE SOUTHERN-OCEAN - EXPERIMENTAL-OBSERVATIONS IN THE WEDDELL AND SCOTIA SEAS
    DEBAAR, HJW
    BUMA, AGJ
    NOLTING, RF
    CADEE, GC
    JACQUES, G
    TREGUER, PJ
    [J]. MARINE ECOLOGY PROGRESS SERIES, 1990, 65 (02) : 105 - 122
  • [6] IMPORTANCE OF IRON FOR PLANKTON BLOOMS AND CARBON-DIOXIDE DRAWDOWN IN THE SOUTHERN-OCEAN
    DEBAAR, HJW
    DEJONG, JTM
    BAKKER, DCE
    LOSCHER, BM
    VETH, C
    BATHMANN, U
    SMETACEK, V
    [J]. NATURE, 1995, 373 (6513) : 412 - 415
  • [7] Falkowski P., 1992, Oceanography, V5, P84, DOI [DOI 10.5670/0CEAN0G.1992.14, DOI 10.5670/OCEANOG.1992.14]
  • [8] VARIATIONS IN CHLOROPHYLL FLUORESCENCE YIELDS IN PHYTOPLANKTON IN THE WORLD OCEANS
    FALKOWSKI, PG
    KOLBER, Z
    [J]. AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1995, 22 (02): : 341 - 355
  • [9] METAL CONTAMINATION AND ITS EFFECT ON PRIMARY PRODUCTION MEASUREMENTS
    FITZWATER, SE
    KNAUER, GA
    MARTIN, JH
    [J]. LIMNOLOGY AND OCEANOGRAPHY, 1982, 27 (03) : 544 - 551
  • [10] GRAN H. H., 1931, Conseil Perm. Internat. pour I'Explor. de la Mer. Rapp. et Proces-Verb., V75, P37