In vitro polarization-resolved resonance Raman studies of the interaction of hematin with the antimalarial drug chloroquine

被引：40

作者：

Frosch, T

Küstner, B

Schlücker, S

Szeghalmi, A

Schmitt, M

Kiefer, W

Popp, J

机构：

[1] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

[2] Univ Wurzburg, Inst Phys Chem, D-97074 Wurzburg, Germany

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2004年 / 35卷 / 10期

关键词：

malaria; hematin-chloroquine complex; polarization-resolved Raman spectroscopy;

D O I：

10.1002/jrs.1252

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Despite the public health impact of human malaria, the mechanism of hemozoin inhibition by antimalarial drugs such as chloroquine (CQ) is not yet fully understood. Polarization-resolved resonance Raman spectra of hematin and its complex with CQ were recorded under Q-band resonance conditions in order to monitor the binding of the 4-aminoquinoline drug to the heme macrocycle. Upon drug addition, only wavenumber shifts smaller than 2 cm(-1) are detected, indicating a non-covalent interaction in the electronic ground state of the drug-target complex. The decreased resonance Raman intensity of the anomalously polarized mode v(19)(a(2g)) in the perpendicular polarized spectrum of the complex suggests changes of the excited-state geometry along the corresponding normal coordinate. Copyright (C) 2004 John Wiley Sons, Ltd.

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页码：819 / 821

页数：3

相关论文

共 11 条

[1] Quinoline binding site on malaria pigment crystal:: A rational pathway for antimalaria drug design [J].

Buller, R ;

Peterson, ML ;

Almarsson, Ö ;

Leiserowitz, L .

CRYSTAL GROWTH & DESIGN, 2002, 2 (06) :553-562

[2] STRUCTURAL CORRELATIONS AND VINYL INFLUENCES IN RESONANCE RAMAN-SPECTRA OF PROTOHEME COMPLEXES AND PROTEINS [J].

CHOI, S ;

SPIRO, TG ;

LANGRY, KC ;

SMITH, KM ;

BUDD, DL ;

LAMAR, GN .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (16) :4345-4351

[3] An assessment of drug-haematin binding as a mechanism for inhibition of haematin polymerisation by quinoline antimalarials [J].

Dorn, A ;

Vippagunta, SR ;

Matile, H ;

Jaquet, C ;

Vennerstrom, JL ;

Ridley, RG .

BIOCHEMICAL PHARMACOLOGY, 1998, 55 (06) :727-736

[4] Structure-function relationships in aminoquinolines:: Effect of amino and chloro groups on quinoline-hematin complex formation, inhibition of β-hematin formation, and antiplasmodial activity [J].

Egan, TJ ;

Hunter, R ;

Kaschula, CH ;

Marques, HM ;

Misplon, A ;

Walden, J .

JOURNAL OF MEDICINAL CHEMISTRY, 2000, 43 (02) :283-291

[5] Characterisation of synthetic β-haematin and effects of the antimalarial drugs quinidine, halofantrine, desbutylhalofantrine and mefloquine on its formation [J].

Egan, TJ ;

Hempelmann, E ;

Mavuso, WW .

JOURNAL OF INORGANIC BIOCHEMISTRY, 1999, 73 (1-2) :101-107

[6] Hemoglobin metabolism in the malaria parasite Plasmodium falciparum [J].

Francis, SE ;

Sullivan, DJ ;

Goldberg, DE .

ANNUAL REVIEW OF MICROBIOLOGY, 1997, 51 :97-123

[7] APPARENT DEPOLARIZATION DISPERSION IN METALLOPORPHYRIN RESONANCE RAMAN-SPECTRA CAN BE AN ARTIFACT OF SPECTRAL CROWDING [J].

HU, SZ ;

SPIRO, TG .

JOURNAL OF PHYSICAL CHEMISTRY, 1995, 99 (18) :7193-7194

[8] RAMAN DIFFERENCE SPECTROSCOPY WITH ROTATING CELL [J].

KIEFER, W .

APPLIED SPECTROSCOPY, 1973, 27 (04) :253-257

[9] Solution structures of antimalarial drug-heme complexes [J].

Leed, A ;

DuBay, K ;

Ursos, LMB ;

Sears, D ;

de Dios, AC ;

Roepe, PD .

BIOCHEMISTRY, 2002, 41 (32) :10245-10255

[10] The structure of malaria pigment β-haematin [J].

Pagola, S ;

Stephens, PW ;

Bohle, DS ;

Kosar, AD ;

Madsen, SK .

NATURE, 2000, 404 (6775) :307-310