Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter

被引：425

作者：

Wood, A

Krogan, NJ

Dover, J

Schneider, J

Heidt, J

Boateng, MA

Dean, K

Golshani, A

Zhang, Y

Greenblatt, JF

Johnston, M

Shilatifard, A

机构：

[1] St Louis Univ, Sch Med, Dept Biochem, St Louis, MO 63104 USA

[2] Univ Toronto, Banting & Best Dept Med Res, Dept Mol & Med Genet, Toronto, ON M5G 1L6, Canada

[3] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA

[4] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA

[5] St Louis Univ, Sch Med, Ctr Canc, St Louis, MO 63104 USA

来源：

MOLECULAR CELL | 2003年 / 11卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(02)00802-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquitination of histone H2B catalyzed by Rad6 is required for methylation of histone H3 by COMPASS. We identified Bre1 as the probable E3 for Rad6's role in transcription. Bre1 contains a C3HC4 (RING) finger and is present with Rad6 in a complex. The RING finger of Bre1 is required for ubiquitination of histone H2B, methylation of lysine 4 and 79 of H3 and for telomeric silencing. Chromatin immunoprecipitation experiments indicated that both Rad6 and Bre1 are recruited to a promoter. Bre1 is essential for this recruitment of Rad6 and is dedicated to the transcriptional pathway of Rad6. These results suggest that Bre1 is the likely E3 enzyme that directs Rad6 to modify chromatin and ultimately to affect gene expression.

引用

页码：267 / 274

页数：8

共 34 条

[1] SPECIFIC COMPLEX-FORMATION BETWEEN YEAST RAD6 AND RAD18 PROTEINS - A POTENTIAL MECHANISM FOR TARGETING RAD6 UBIQUITIN-CONJUGATING ACTIVITY TO DNA-DAMAGE SITES
BAILLY, V
LAMB, J
SUNG, P
PRAKASH, S
PRAKASH, L
[J]. GENES & DEVELOPMENT, 1994, 8 (07) : 811 - 820
[2] Gene silencing -: Trans-histone regulatory pathway in chromatin
Briggs, SD
Xiao, TJ
Sun, ZW
Caldwell, JA
Shabanowitz, J
Hunt, DF
Allis, CD
Strahl, BD
[J]. NATURE, 2002, 418 (6897) : 498 - 498
[3] Gene expression - Emerging roles of ubiquitin in transcription regulation
Conaway, RC
Brower, CS
Conaway, JW
[J]. SCIENCE, 2002, 296 (5571) : 1254 - 1258
[4] Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6
Dover, J
Schneider, J
Tawiah-Boateng, MA
Wood, A
Dean, K
Johnston, M
Shilatifard, A
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (32) : 28368 - 28371
[5] A Saccharomyces cerevisiae phleomycin sensitive mutant, ph140, is defective in the RAD6 DNA repair gene
He, CH
Masson, JY
Ramotar, D
[J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1996, 42 (12) : 1263 - 1266
[6] REGULATION OF THE SACCHAROMYCES-CEREVISIAE SRS2 HELICASE DURING THE MITOTIC CELL-CYCLE, MEIOSIS AND AFTER IRRADIATION
HEUDE, M
CHANET, R
FABRE, F
[J]. MOLECULAR & GENERAL GENETICS, 1995, 248 (01): : 59 - 68
[7] Saccharomyces cerevisiae MGS1 is essential in strains deficient in the RAD6-dependent DNA damage tolerance pathway
Hishida, T
Ohno, T
Iwasaki, H
Shinagawa, H
[J]. EMBO JOURNAL, 2002, 21 (08) : 2019 - 2029
[8] Biochemistry - All in the ubiquitin family
Hochstrasser, M
[J]. SCIENCE, 2000, 289 (5479) : 563 - 564
[9] The ubiquitin-conjugating enzyme Rad6 (Ubc2) is required for silencing in Saccharomyces cerevisiae
Huang, HH
Kahana, A
Gottschling, DE
Prakash, L
Liebman, SW
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (11) : 6693 - 6699
[10] THE YEAST DNA-REPAIR GENE RAD6 ENCODES A UBIQUITIN-CONJUGATING ENZYME
JENTSCH, S
MCGRATH, JP
VARSHAVSKY, A
[J]. NATURE, 1987, 329 (6135) : 131 - 134

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