Quantitation of membrane type serine protease 1 (MT-SP1) in transformed and normal cells

被引:29
作者
Bhatt, AS
Takeuchi, T
Ylstra, B
Ginzinger, D
Albertson, D
Shuman, MA
Craik, CS
机构
[1] Univ Calif San Francisco, Sch Med, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Ctr Comprehens Canc, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
[4] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA
基金
美国国家卫生研究院;
关键词
breast; cancer; MT-SP1; protease; serine;
D O I
10.1515/BC.2003.029
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Membrane type serine protease 1 (MT-SP1) is a representative member of a large family of related enzymes known as type II transmembrane serine proteases or membrane type serine proteases. MT-SP1 has been implicated in the selective proteolysis of key extracellular substrates but its physiological role is still not fully understood. MT-SP1 expression at the protein and RNA level has been previously examined by nonquantitative methods such as in situ hybridization, Northern blotting and immunohistochemistry. To establish an introductory understanding of the quantitative mRNA expression of MT-SP1 and to correlate these levels with urokinase-type plasminogen activator receptor (uPAR), a key component of extracellular proteolysis, quantitative RT-PCR was carried out. RNA expression was analyzed in 34 human cancer cell lines, 26 human tissues and 18 primary human breast cancer tissue samples. MT-SP1 mRNA is highly expressed in many breast, ovarian, prostate and colon cancer cell lines and normal human tissues of endodermal origin. At the transcript level, MT-SP1 shows a highly statistically significant correlation (Pearsons product moment correlation r = 0.784, p < 0.001) with uPAR in human breast cancer tissue. The exact role of MT-SP1 in concert with proteins such as uPAR and other members of the plasminogen activator cascade has yet to be ascertained. However, the significant correlation between MT-SP1 and uPAR transcript levels in this initial study suggests further work to establish the role of MT-SP1 as a possible prognostic, diagnostic or therapeutic target for breast cancer.
引用
收藏
页码:257 / 266
页数:10
相关论文
共 43 条
  • [1] Bell William R., 1996, Seminars in Thrombosis and Hemostasis, V22, P459
  • [2] Influence of the extraction procedure on plasminogen activator inhibitor-2 (PAI-2) and urokinase receptor (uPAR) assays in breast cancer tissues
    BouchetBernet, C
    Spyratos, F
    Andrieu, C
    Deytieux, S
    Becette, V
    Oglobine, J
    [J]. BREAST CANCER RESEARCH AND TREATMENT, 1996, 41 (02) : 141 - 146
  • [3] Prognostic value of uPA and p53 accumulation measured by quantitative biochemical assays in 1245 primary breast cancer patients:: a multicentre study
    Broët, P
    Spyratos, F
    Romain, S
    Quillien, V
    Daver, A
    Ricolleau, G
    Rallet, A
    Toulas, C
    Asselain, B
    [J]. BRITISH JOURNAL OF CANCER, 1999, 80 (3-4) : 536 - 545
  • [4] Cao J, 2001, CHINESE MED J-PEKING, V114, P726
  • [5] Prognostic impact of urokinase-type plasminogen activator receptor (uPAR) in cytosols and pellet extracts derived from primary breast tumours
    de Witte, JH
    Foekens, JA
    Brünner, N
    Heuvel, JJTM
    van Tienoven, TH
    Look, MP
    Klijn, JGM
    Geurts-Moespot, A
    Grebenchtchikov, N
    Benraad, TJ
    Sweep, CGJ
    [J]. BRITISH JOURNAL OF CANCER, 2001, 85 (01) : 85 - 92
  • [6] DELVECCHIO S, 1993, CANCER RES, V53, P3198
  • [7] Delineation of prognostic biomarkers in prostate cancer
    Dhanasekaran, SM
    Barrette, TR
    Ghosh, D
    Shah, R
    Varambally, S
    Kurachi, K
    Pienta, KJ
    Rubin, MA
    Chinnaiyan, AM
    [J]. NATURE, 2001, 412 (6849) : 822 - 826
  • [8] Duffy M J, 1990, Blood Coagul Fibrinolysis, V1, P681
  • [9] Structure-based approach for the discovery of bis-benzamidines as novel inhibitors of matriptase
    Enyedy, IJ
    Lee, SL
    Kuo, AH
    Dickson, RB
    Lin, CY
    Wang, SM
    [J]. JOURNAL OF MEDICINAL CHEMISTRY, 2001, 44 (09) : 1349 - 1355
  • [10] Foekens JA, 2000, CANCER RES, V60, P636