Uptake and intracellular activity of ofloxacin isomers in human phagocytic and non-phagocytic cells

被引：19

作者：

García, I ^{[1
]}

Pascual, A ^{[1
]}

Ballesta, S ^{[1
]}

Perea, EJ ^{[1
]}

机构：

[1] Sch Med, Dept Microbiol, Seville, Spain

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2000年 / 15卷 / 03期

关键词：

intracellular penetration; fluoroquinolones; phagocytes; ofloxacin;

D O I：

10.1016/S0924-8579(00)00161-8

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The penetration and intracellular activity of ofloxacin and its isomers (levofloxacin and D-ofloxacin) into human polymorphonuclear leucocytes (PMN), human peritoneal macrophages (PM phi) and tissue cultured epithelial cells (McCoy) were evaluated. The cellular to extracellular concentration (C/E) values of the three fluoroquinolones were higher than 3.6 and 2.6 in PMN and PM phi, respectively. The C/E ratios in McCoy cells were lower than those in PMN, but still higher than 2.0. The uptake of ofloxacin and its isomers was rapid, non-saturable and reversible. All quinolones (extracellular concentrations: 2, 5 and 10 mg/l) produced a significant reduction of viable intraphagocytic Staphylococcus anl eus in phagocytic cells. We concluded that ofloxacin and its isomers reach high intracellular concentrations in phagocytic and Iron phagocytic cells while remaining active in the former. (C) 2000 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.

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页码：201 / 205

页数：5

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