Effects of ozone and oxygen on the degradation of carotenoids in an aqueous model system

被引:82
作者
Henry, LK
Puspitasari-Nienaber, NL
Jarén-Galán, M
van Breemen, RB
Catignani, GL
Schwartz, SJ [1 ]
机构
[1] Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA
[2] N Carolina State Univ, Dept Food Sci, Raleigh, NC 27695 USA
[3] CSIC, Inst Grasa & Derivados, E-41012 Seville, Spain
[4] Univ Illinois, Dept Med Chem & Pharmacognosy, Chicago, IL 60612 USA
关键词
ozone; oxidation; carotenoids; beta-carotene; beta-cryptoxanthin; lycopene; degradation;
D O I
10.1021/jf000503o
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The effects of ozone and oxygen on the degradation of carotenoids in an aqueous model system were studied. All-trans beta -carotene, 9-cis beta -carotene, beta -cryptoxanthin, and lycopene were adsorbed onto a C-18 solid phase and exposed to a continuous flow of water saturated with oxygen or ozone at 30 degreesC. Carotenoids were analyzed using HPLC with a C-30 column and a photodiode array detector. Approximately 90% of all-trans beta -carotene, 9-cis beta -carotene, and beta -cryptoxanthin were lost after exposure to ozone for 7 h. A similar loss of lycopene occurred in only 1 h. When exposed to oxygen, all carotenoids, except beta -cryptoxanthin, degraded at lower rates. The degradation of all the carotenoids followed zero-order reaction kinetics with the following relative rates: lycopene > beta -cryptoxanthin > all-trans beta -carotene > 9-cis beta -carotene. The major degradation products of-carotene were tentatively identified on the basis of their elution on the HPLC column, UV-Vis spectra, and electrospray LC-MS. Predominant isomers of beta -carotene were 13-cis, 9-cis, and a di-cis isomer. Products resulting from cleavage of the molecule were beta -apo-13-carotenone and beta -apo-14'-carotenal, whereas epoxidation yielded beta -carotene 5,8-epoxide and beta -carotene 5,8-endoperoxide.
引用
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页码:5008 / 5013
页数:6
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