Hepatocyte growth factor scatter factor activates the apoptosis signaling pathway by increasing caspase-3 activity in Sarcoma 180 cells

被引：37

作者：

Arakaki, N ^{[1
]}

Kazi, JA ^{[1
]}

Kazihara, T ^{[1
]}

Ohnishi, T ^{[1
]}

Daikuhara, Y ^{[1
]}

机构：

[1] Kagoshima Univ, Sch Dent, Dept Biochem, Kagoshima 8908544, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 245卷 / 01期

关键词：

D O I：

10.1006/bbrc.1998.8397

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hepatocyte growth factor, which is now known to be the same protein as scatter factor, induced oligonucleosomal fragmentation of nuclear DNA of Sarcoma 180 cells and increased the activity of caspase-3, a key component in control of the apoptotic cell death pathway to about 2.6 times that in control cells on 48 hr incubation, but did not increase the activity of caspase-1. Both HGF-induced DNA fragmentation and caspase-3 activity were completely inhibited by co-incubation with an inhibitor of caspase-3, Ac-DEVD-H. In contrast, HGF did not affect the expression of the apoptosis suppressors Bcl-2 and Bcl-x, These results indicate that HGF activates the apoptosis signaling pathway by increasing caspase-3 activity in Sarcoma 180 cells. (C) 1998 Academic Press.

引用

页码：211 / 215

页数：5

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