Half-genome human immunodeficiency virus type 1 constructs for rapid production of reporter viruses

被引：23

作者：

Ali, A

Jamieson, BD

Yang, OO

机构：

[1] Univ Calif Los Angeles, Med Ctr, Dept Med, Div Infect Dis, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Med Ctr, AIDS Inst, Los Angeles, CA 90095 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2003年 / 110卷 / 02期

关键词：

HIV-1; reporter viruses; flow cytometry;

D O I：

10.1016/S0166-0934(03)00110-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of reporter constructs of HIV-1 molecular clones has been useful in studying the effects of infection on individual cells. A strategy of producing viral half-genome constructs containing reporter genes that can be used to produce recombinant complete infectious virions is described. This approach allows rapid generation of reporter and non-reporter virions from the same genetic construct, obviating the need to produce a new reporter genetic construct for each viral change to be studied. We provide an example of the utility of this system for studying the effects of Nef on infected cells. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：137 / 142

页数：6

共 12 条

[1] PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE [J].