Induction of hemolin gene expression by bacterial cell wall components in eri-silkworm, Samia cynthia ricini

被引:17
作者
Bao, Yanyuan [1 ]
Yamano, Yoshiaki [1 ]
Morishima, Isao [1 ]
机构
[1] Tottori Univ, Fac Agr, Dept Biochem & Biotechnol, Tottori 6808553, Japan
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2007年 / 146卷 / 01期
关键词
insect immunity; immune-induced gene; hemolin; eri-silkworm; Samia cynthia ricini; peptidoglycan;
D O I
10.1016/j.cbpb.2006.10.092
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA clone encoding hemolin was isolated from fat body of immunized Samia cynthia ricini larvae based on subtractive suppression hybridization method. The cDNA encodes 413 amino acid residue open reading frame with an 18 residue predicted signal peptide. The expression of the gene was strongly induced in fat body and midgut by an injection of bacterial cells or peptidoglycans, but very weakly by lipopolysaccharide. The mRNA expression in the fat body was detected as early as 3 h post-injection, and reached the peak level at 12 h. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:147 / 151
页数:5
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