Adhesion of Salmonella enterica serotype Enteritidis isolates to chicken isthmal glandular secretions

被引:33
作者
De Buck, J [1 ]
Van Immerseel, F [1 ]
Meulemans, G [1 ]
Haesebrouck, F [1 ]
Ducatelle, R [1 ]
机构
[1] Univ Ghent, Fac Med Vet, Dept Pathol Bacteriol & Avian Dis, B-9820 Merelbeke, Belgium
关键词
Salmonella Enteritidis; chicken-bacteria; oviduct; fimbriae; mannose; isthmus; egg;
D O I
10.1016/S0378-1135(03)00038-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The ability of Salmonella enterica serotype Enteritidis isolates to adhere to immobilized secretions of the isthmus of the laying hen was determined in an ELISA-type assay. One-third of the 56 isolates tested in the logarithmic growth phase, adhered to the isthmal secretions. Using a binding assay of the isolates to thin paraffin sections of the oviduct, we demonstrated that the receptor of the adhesion was localized inside the tubular gland cells of the isthmus. The adhesion to immobilized isthmal secretions as well as to the paraffin sections was blocked by the addition of mannose. A fimD mutant of S. Enteritidis, lacking type 1 fimbriae, did not adhere, confirming that the adhesion was mediated by type 1 fimbriae. Mannosylated glycoproteins were demonstrated in the isthmus glandular cells using confocal laser scanning microscopy by FITC-labelled Lens culinaris lectins. It is hypothesized that the binding of S. Enteritidis to isthmal secretions could play a role in the contamination of eggs through incorporation of the bacteria in the shell membranes. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:223 / 233
页数:11
相关论文
共 35 条
[1]   The role of flagella, but not fimbriae, in the adherence of Salmonella enterica serotype Enteritidis to chick gut explant [J].
Allen-Vercoe, E ;
Woodward, MJ .
JOURNAL OF MEDICAL MICROBIOLOGY, 1999, 48 (08) :771-780
[2]  
BAUMLER AJ, 1995, J BACTERIOL, V177, P2087
[3]  
Bichler LA, 1996, AM J VET RES, V57, P489
[4]   LOCATION OF SALMONELLA-TYPHIMURIUM DURING INCUBATION AND HATCHING OF INOCULATED EGGS [J].
CASON, JA ;
BAILEY, JS ;
COX, NA .
POULTRY SCIENCE, 1993, 72 (11) :2064-2068
[5]   Detection of antibodies to Salmonella enteritidis in sera and yolks from experimentally and naturally infected chickens [J].
Desmidt, M ;
Ducatelle, R ;
Haesebrouck, F ;
DeGroot, PA ;
Verlinden, M ;
Wijffels, R ;
Hinton, M ;
Bale, JA ;
Allen, VM .
VETERINARY RECORD, 1996, 138 (10) :223-226
[6]   Role of the humoral immune system in Salmonella enteritidis phage type four infection in chickens [J].
Desmidt, M ;
Ducatelle, R ;
Mast, J ;
Goddeeris, BM ;
Kaspers, B ;
Haesebrouck, F .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1998, 63 (04) :355-367
[7]   FIMBRIAE AND ADHESIVE PROPERTIES IN SALMONELLAE [J].
DUGUID, JP ;
ANDERSON, ES ;
CAMPBELL, I .
JOURNAL OF PATHOLOGY AND BACTERIOLOGY, 1966, 92 (01) :107-&
[8]  
ETCHES RJ, 1996, REPROD POULTRY, P173
[9]   PRODUCTION OF SALMONELLA-ENTERITIDIS-CONTAMINATED EGGS BY EXPERIMENTALLY INFECTED HENS [J].
GAST, RK ;
BEARD, CW .
AVIAN DISEASES, 1990, 34 (02) :438-446
[10]  
HALD T, 1999, ANN REP ZOONOSES DEN, P18