Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis

被引：24

作者：

Agarwal, Shivangi ^{[1
]}

Agarwal, Shivani ^{[1
]}

Bhatnagar, Rakesh ^{[1
]}

机构：

[1] Jawaharlal Nehru Univ, Sch Biotechnol, Gene Regulat Lab, New Delhi 110067, India

来源：

FEBS LETTERS | 2007年 / 581卷 / 09期

关键词：

toxin-antitoxin locus; addiction module; post segregational killing; plasmid inheritance; Bacillus anthracis;

D O I：

10.1016/j.febslet.2007.03.051

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparative genome analysis of Bacillus anthracis revealed a pair of linked genes encoding pemK (K, killer protein) and pemI (I, inhibitory protein) homologous to pem loci of other organisms. Expression of PemK in Escherichia coli and Bacillus anthracis was bacteriostatic whereas the concomitant expression of PemI reversed the growth arrest. PemK expression effectively inhibited protein synthesis with no significant effect on DNA replication. Coexpression and interaction of these proteins confirmed it to be a Type II addiction module. Thermal denaturation analysis reflected poor conformational stability of PemI as compared to PemK. Circular dichroism analysis indicated that PemI contains twice the amount of beta-sheets as PemK. Gel retardation assays demonstrated that PemI binds to its upstream DNA sequence. This study reports the first evidence of an active chromosome encoded toxin-antitoxin locus in B. anthracis. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1727 / 1734

页数：8

共 36 条

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