Transcription factor nuclear factor κB regulates the inducible expression of the human B1 receptor gene in inflammation

Expression of the bradykinin B-1 receptor gene is upregulated in vascular smooth muscle cells (VSMCs) in response to a variety of inflammatory stimuli. We isolated the 5'-flanking region of the human bradykinin B-1 receptor gene and examined its promoter activity by transient transfection analysis. This region (-2582 to +34) showed promoter activity inducible by lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) in VSMCs. Further deletion analysis revealed that constructs containing 111 base pairs of 5'-flanking sequence were sufficient for transcriptional induction. Mutagenesis of a nuclear factor ICE (NF-KB)like site at -64 to -55 abolished most of the LPS, TNF-alpha; and IL-1 beta inducibility, whereas a mutation of a cyclic AMP response element at -50 to -43 markedly reduced the basal promoter activity, and a mutation of the activator protein 1 (AP-1) site at -78 to -72 had minimal effects. Nuclear extracts from LPS, TNF-alpha, and IL-1 beta-treated VSMCs, IL-1 beta-treated human hepatoma HepG(2), and human lung fibroblast IMR-90 cells showed strong. inducible binding activity to the NF-kappa B-like site by gel shift assays. These results demonstrated that NF-kappa B-like nuclear factor was involved in the inducible expression of the human bradykinin B-1 receptor gene during inflammatory processes.