Translocation properties of novel cell penetrating transportan and penetratin analogues

被引：88

作者：

Lindgren, M

Gallet, X

Soomets, U

Hällbrink, M

Bråkenhielm, E

Pooga, M

Brasseur, R

Langel, Ü ^{[1
]}

机构：

[1] Univ Stockholm, Arrhenius Lab, Dept Neurochem & Neurotoxicol, S-10691 Stockholm, Sweden

[2] Univ Gembloux, Fac Agron, Ctr Biophys Mol Numer, B-5030 Gembloux, Belgium

[3] Univ Tartu, Dept Biochem, EE-50411 Tartu, Estonia

[4] Estonian Bioctr, EE-51010 Tartu, Estonia

来源：

BIOCONJUGATE CHEMISTRY | 2000年 / 11卷 / 05期

关键词：

D O I：

10.1021/bc990156s

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel analogues of the cell-penetrating peptides penetratin and transportan were synthesized. The distribution of the biotin-labeled peptides in Bowes melanoma cell line has been investigated by indirect fluorescence with fluorescein-streptavidin detection. The time course of uptake of I-125-labeled transportan analogues has been characterized in the same cell line. Molecular modeling was used to analyze the penetration and the orientation of molecules in a simulated biological membrane. The results, both from molecular modeling and fluorescence studies, imply that penetratin and transportan do not enter the cells by related mechanisms and that they do not belong to the same family of translocating peptides.

引用

页码：619 / 626

页数：8

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