Evaluation of the BDProbeTec ET system for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples:: a multicenter study

被引：28

作者：

Mazzarelli, G

Rindi, L

Piccoli, P

Scarparo, C

Garzelli, C

Tortoli, E

机构：

[1] Careggi Hosp, Reg Reference Ctr Mycobacteria, Microbiol & Virol Lab, Piastra Serv, I-50134 Florence, Italy

[2] Careggi Hosp, Microbiol & Virol Serum Immunol Lab, I-50134 Florence, Italy

[3] Univ Pisa, Dept Expt Pathol Med Biotechnol Infectivol & Epid, Pisa, Italy

[4] San Bortolo Hosp, Reg Reference Ctr Mycobacteria, Vicenza, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 04期

关键词：

D O I：

10.1128/JCM.41.4.1779-1782.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated the BDProbeTec ET system (Becton Dickinson, Sparks, Md.), a strand displacement amplification-based technique, for direct detection of Mycobacterium tuberculosis in 867 clinical samples. Of 294 extrapulmonary specimens, 52 had positive results by both BDProbeTec ET and culture and 209 had negative results by both methods; sensitivity and specificity were 76.5 and 95.9%, respectively. After resolution of discrepancies, the sensitivity rose to 77.8%.

引用

页码：1779 / 1782

页数：4

共 26 条

[1] Clinical evaluation of the BDProbeTec strand displacement amplification assay for rapid diagnosis of tuberculosis [J].

Bergmann, JS ;

Woods, GL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (09) :2766-2768

[2] Clinical evaluation of the BDProbeTec ET system for rapid detection of Mycobacterium tuberculosis [J].

Bergmann, JS ;

Keating, WE ;

Woods, GL .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (02) :863-865

[3] DIAGNOSIS OF TUBERCULOSIS BY AMPLICOR MYCOBACTERIUM-TUBERCULOSIS TEST - A MULTICENTER STUDY [J].

CARPENTIER, E ;

DROUILLARD, B ;

DAILLOUX, M ;

MOINARD, D ;

VALLEE, E ;

DUTILH, B ;

MAUGEIN, J ;

BERGOGNEBEREZIN, E ;

CARBONNELLE, B .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (12) :3106-3110

[4] Standing statistics right side up [J].

Davidoff, F .

ANNALS OF INTERNAL MEDICINE, 1999, 130 (12) :1019-1021

[5] Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by gen-probe amplified Mycobacterium tuberculosis direct test [J].

Gamboa, F ;

Manterola, JM ;

Vinado, B ;

Matas, L ;

Gimenez, M ;

Lonca, J ;

Manzano, JR ;

Rodrigo, C ;

Cardona, PJ ;

Padilla, E ;

Dominguez, J ;

Ausina, V .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (01) :307-310

[6] Comparative evaluation of initial and new versions of the Gen-Probe amplified Mycobacterium Tuberculosis Direct Test for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens [J].

Gamboa, F ;

Fernandez, G ;

Padilla, E ;

Manterola, JM ;

Lonca, J ;

Cardona, PJ ;

Matas, L ;

Ausina, V .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (03) :684-689

[7] Evaluation of the Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in human samples [J].

Garrino, MG ;

Glupczynski, Y ;

Degraux, J ;

Nizet, H ;

Delmée, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (01) :229-232

[8] Diagnostic value of the strand displacement amplification method compared to those of Roche Amplicor PCR and culture for detecting mycobacteria in sputum samples [J].

Ichiyama, S ;

Ito, Y ;

Sugiura, F ;

Iinuma, Y ;

Yamori, S ;

Shimojima, M ;

Hasegawa, Y ;

Shimokata, K ;

Nakashima, N .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (12) :3082-3085

[9] Evaluation of a new commercial assay for diagnosis of pulmonary and nonpulmonary tuberculosis [J].

Johansen, IS ;

Thomsen, VO ;

Johansen, A ;

Andersen, P ;

Lundgren, B .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2002, 21 (06) :455-460

[10]

Little MC, 1999, CLIN CHEM, V45, P777

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