Stage-specific expression of the 14-3-3 gene in Echinococcus multilocularis

被引:52
作者
Siles-Lucas, M
Felleisen, RSJ
Hemphill, A
Wilson, W
Gottstein, B
机构
[1] Univ Bern, Inst Parasitol, CH-3012 Bern, Switzerland
[2] Univ Bern, Inst Gen Microbiol, CH-3012 Bern, Switzerland
关键词
Echinococcus multilocularis; 14-3-3; protein; metacestode; germinal layer; Saccharomyces cerevisiae complementation;
D O I
10.1016/S0166-6851(97)00208-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA expression library representing the metacestode developmental stage of the tapeworm Echinococcus multilocularis was immunoscreened with monospecific antibodies affinity purified following differential immunoblot analysis. Using this procedure, a metacestode-specific clone was isolated representing a 14-3-3 gene of the parasite, which is present as a single copy in the parasite genome. The identity of this clone was demonstrated by cross-reactivity of the recombinant E. multilocularis 14-3-3 protein with antibodies raised against a heterologous 14-3-3 protein from Saccharomyces cerevisiae. In addition, expression of the E. multilocularis 14-3-3 gene in the mutant S. cerevisiae strain, DS9-22, resulted in complementation of the phenotypic deficiency of this strain, thus demonstrating the functionality of the respective gene product. By reverse transcription-polymerase chain reaction (RT-PCR) we showed that the E. multilocularis 14-3-3 protein is about 10-fold overexpressed in the metacestode stage compared with the expression level in the adult stage. Immunolocalization of the 14-3-3 protein in E. multilocularis metacestodes revealed its predominant presence in the germinal layer of the parasite. The results of this study, taken together with the current knowledge on the 14-3-3 protein family, suggest that this parasite molecule may contribute to the promotion of the progessive, potentially unlimited growth behaviour of the E. multilocularis metacestode within the host tissue. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:281 / 293
页数:13
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