Differential light-scattering spectroscopy: a new approach to studying of colloidal gold nanosensors

被引：38

作者：

Khlebtsov, NG ^{[1
]}

Bogatyrev, VA ^{[1
]}

Melnikov, AG ^{[1
]}

Dykman, LA ^{[1
]}

Khlebtsov, BN ^{[1
]}

Krasnov, YM ^{[1
]}

机构：

[1] RAS, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410049, Russia

来源：

JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER | 2004年 / 89卷 / 1-4期

基金：

俄罗斯基础研究基金会;

关键词：

gold nanoparticles; bioconjugates; aggregation; resonance light scattering; plasmon resonance; T-matrix method;

D O I：

10.1016/j.jqsrt.2004.05.017

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Addition of complementary components to a bioconjugate probe results in aggregation of nanoparticles that can be monitored by extinction spectra. This is the core idea of the well-known sol particle immunoassay (SPIA) introduced by Leuvering et al. (J. Immunoassay 1 (1980) 77). Here, we describe a new approach to study the biospecific interactions in systems of colloidal gold bioconjugates. The method is based on measuring static light-scattering spectra (at 90degrees) within a wavelength range of 350-800 nm. To this end, we have developed a special attachment to the Specord M-40 spectrophotometer and a corresponding measurement procedure called by us "differential light-scattering spectroscopy" (DLSS). The DLSS technique has been compared with conventional spectrophotometry as applied to colloidal gold conjugates with various biopolymers. Our theoretical simulations and experiments with gold particles of various sizes showed a higher potential sensitivity of the method proposed as compared with conventional extinction spectroscopy. It is expected that DLSS can be used to develop an analytical biospecific test for various biopolymers. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：133 / 142

页数：10

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