Tissue-engineered bone regeneration

被引：1173

作者：

Petite, H ^{[1
]}

Viateau, V ^{[1
]}

Bensaïd, W ^{[1
]}

Meunier, A ^{[1
]}

de Pollak, C ^{[1
]}

Bourguignon, M ^{[1
]}

Oudina, K ^{[1
]}

Sedel, L ^{[1
]}

Guillemin, G ^{[1
]}

机构：

[1] Univ D Diderot, Fac Med Lariboisiere St Louis, CNRS UPRES A 7052, Lab Rech Orthoped, F-75010 Paris, France

来源：

NATURE BIOTECHNOLOGY | 2000年 / 18卷 / 09期

关键词：

tissue engineering; mesenchymal stem cells; bone repair; marrow stromal cells; coral;

D O I：

10.1038/79449

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bone lesions above a critical size become scarred rather than regenerated, leading to nonunion. We have attempted to obtain a greater degree of regeneration by using a resorbable scaffold with regeneration-competent cells to recreate an embryonic environment in injured adult tissues, and thus improve clinical outcome, We have used a combination of a coral scaffold with in vitro-expanded marrow stromal cells (MSC) to increase osteogenesis more than that obtained with the scaffold alone or the scaffold plus fresh bone marrow, The efficiency of the various combinations was assessed in a large segmental defect model in sheep, The tissue-engineered artificial bone underwent morphogenesis leading to complete recorticalization and the formation of a medullary canal with mature lamellar cortical bone in the most favorable cases. Clinical union never occurred when the defects were left empty or filled with the scaffold alone, In contrast, clinical union was obtained in three out of seven operated limbs when the defects were filled with the tissue-engineered bone.

引用

页码：959 / 963

页数：5

共 36 条

[1] Bone regeneration by implantation of purified, culture-expanded human mesenchymal stem cells [J].