Ring-like pore structures of SecA: Implication for bacterial protein-conducting channels

被引:64
作者
Wang, HW
Chen, Y
Yang, HC
Chen, XC
Duan, MX
Tai, PC
Sui, SF
机构
[1] Georgia State Univ, Dept Biol, Atlanta, GA 30303 USA
[2] Tsinghua Univ, Dept Biol Sci & Biotechnol, State Key Lab Biomembranes, Beijing 100084, Peoples R China
关键词
D O I
10.1073/pnas.0737415100
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
SecA, an essential component of the general protein secretion pathway of bacteria, is present in Escherichia coli as soluble and membrane-integral forms. Here we show by electron microscopy that SecA assumes two characteristic forms in the presence of phospholipid monolayers: dumbbell-shaped elongated structures and ring-like pore structures. The ring-like pore structures with diameters of 8 nm and holes of 2 nm are found only in the presence of anionic phospholipids. These ring-like pore structures with larger 3- to 6-nm holes (without staining) were also observed by atomic force microscopic examination. They do not form in solution or in the presence of uncharged phosphatidylcholine. These ring-like phospholipid-induced pore-structures may form the core of bacterial protein-conducting channels through bacterial membranes.
引用
收藏
页码:4221 / 4226
页数:6
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