Monitoring neural activity with bioluminescence during natural behavior

被引：139

作者：

Naumann, Eva A. ^{[1
]}

Kampff, Adam R. ^{[1
]}

Prober, David A. ^{[2
]}

Schier, Alexander F. ^{[1
]}

Engert, Florian ^{[1
]}

机构：

[1] Harvard Univ, Ctr Brain Sci, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[2] CALTECH, Div Biol, Pasadena, CA 91125 USA

来源：

NATURE NEUROSCIENCE | 2010年 / 13卷 / 04期

基金：

美国国家卫生研究院;

关键词：

IN-VIVO; ZEBRAFISH; CA2+; AEQUORIN; SLEEP; HYPOCRETIN; NEURONS; SINGLE; BRAIN; VISUALIZATION;

D O I：

10.1038/nn.2518

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Existing techniques for monitoring neural activity in awake, freely behaving vertebrates are invasive and difficult to target to genetically identified neurons. We used bioluminescence to non-invasively monitor the activity of genetically specified neurons in freely behaving zebrafish. Transgenic fish with the Ca2+-sensitive photoprotein green fluorescent protein (GFP)-Aequorin in most neurons generated large and fast bioluminescent signals that were related to neural activity, neuroluminescence, which could be recorded continuously for many days. To test the limits of this technique, we specifically targeted GFP-Aequorin to the hypocretin-positive neurons of the hypothalamus. We found that neuroluminescence generated by this group of similar to 20 neurons was associated with periods of increased locomotor activity and identified two classes of neural activity corresponding to distinct swim latencies. Our neuroluminescence assay can report, with high temporal resolution and sensitivity, the activity of small subsets of neurons during unrestrained behavior.

引用

页码：513 / U146

页数：10

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[1] Neural substrates of awakening probed with optogenetic control of hypocretin neurons [J].