Immunocytochemical characterization of in vitro PKH26-labelled and intracerebrally transplanted neonatal cells

被引：46

作者：

Haas, SJP

Bauer, P

Rolfs, A

Wree, A

机构：

[1] Univ Rostock, Inst Anat, D-18055 Rostock, Germany

[2] Univ Rostock, Dept Neurol, D-18055 Rostock, Germany

来源：

ACTA HISTOCHEMICA | 2000年 / 102卷 / 03期

关键词：

PKH26; fluorescent dye; freeze-thawing; transplantation; intracerebral graft; immunocytochemistry;

D O I：

10.1078/S0065-1281(04)70035-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The lipophilic dye PKH26 that binds irreversibly to cell membranes has been used to label various cell types in vitro prior to transplantation in order to recognize grafted cells posttransplantationally in the host tissue by fluorescence microscopy. The purpose of the present study was to optimize immunocytochemical staining procedures for PKH26-containing specimens in cell culture and after transplantation into rat brain. We demonstrated that freeze-thawing allowed for proper immunostaining of intracellular epitopes whereas PKH26-labelling was preserved. PKH26-labelled donor cells were detectable at least up to 4 months after transplantation in the host brain.

引用

页码：273 / 280

页数：8

共 26 条

[1] Amelioration of ischaemia-induced neuronal death in the rat striatum by NGF-secreting neural stem cells [J].