Donor nutritional status - A determinant of liver preservation injury

In liver transplantation, the quality of the liver is determined by a number of factors including donor nutritional status, Livers from fasted donors appear to tolerate long-term preservation better than livers from fed donors, In this study we repeated earlier results and obtained 31% (4/13) survival after 40-hr preservation of livers from fed donor Brown Norway rats and 67% (8/12) survivors with donor livers from 4-day-fasted rats (P=0.154), The explanation for this improvement is not known but may be due to inactivation of Kupffer cells due to nutritional depletion of the liver, Kupffer cell activation has been one explanation advanced to explain how cold storage injures livers during reperfusion (transplantation). In this study, we have measured how donor fasting affects Kupffer cell function (phagocytosis of colloidal carbon) after preservation of the rat liver, In addition, we measured how enhancing liver glycogen by feeding glucose to the rat donors affected outcome and liver functions tested by isolated perfusion after 24- and 40-hr cold storage of the liver, Preservation did not cause inactivation or activation of Kupffer cell phagocytosis of colloidal carbon, In livers with 0-hr preservation, colloidal carbon uptake was 3.1+/-0.2 mg/g/hr, after 40-hr preservation uptake was 3.8 mg/g/hr (P<0.05 vs. 0 hr) (fed) and 2.7+/-0.3 mg/g/hr (fasted, P, 0.05 vs. 0-hr and 40-hr-fed), Thus, the improved survival obtained with livers from fasted donors does not appear related to inactivation of Kupffer cell phagocytosis, Although livers from fasted donors showed improved survival, there was extensive hepatocellular injury as indicated by large LDH release from the livers after 40-hr cold storage as tested by isolated perfusion, LDH released into the perfusate increased from 35.8+/-10.1 U/L (fed, 40-hr CS) to 301+/-65 U/L (fasted, 40-hr CS) after 1-hr reperfusion, AST release showed a similar pattern and bile production was suppressed more in livers from fasted donors than fed donors, Feeding rats glucose elevated liver glycogen and significantly reduced hepatocellular injury as measured by LDH release and AST release in the isolated perfused liver after 40-hr cold storage, Feeding rats glucose (40% in drinking water for 4 days) also improved survival: fed + glucose = 85% survival versus 31% survival with no glucose and fasted + glucose = 92% survival versus 67% survival with no glucose. These results show that both extensive donor fasting and glucose feeding enhanced outcome in orthotopic liver transplantation. This dilemma (both fasting and feeding improved survival) are discussed in terms of how the interactions between Kupffer cells and hepatocytes affect liver viability. Donor fasting is probably impractical clinically as a method to improve the donor liver, but elevating liver glycogen by glucose supplementation is possible and may lead to improved preservation and outcome in liver transplantation.