Molecular cloning and characterization of a metal responsive Aedes aegypti intestinal mucin cDNA

被引：41

作者：

Rayms-Keller, A ^{[1
]}

McGaw, M ^{[1
]}

Oray, C ^{[1
]}

Carlson, JO ^{[1
]}

Beaty, BJ ^{[1
]}

机构：

[1] Colorado State Univ, Dept Microbiol, Ctr Environm Toxicol & Technol, Arthropod Borne & Infect Dis Lab, Ft Collins, CO 80523 USA

来源：

INSECT MOLECULAR BIOLOGY | 2000年 / 9卷 / 04期

关键词：

D O I：

10.1046/j.1365-2583.2000.00202.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a cDNA from Aedes aegypti that is transcribed in the larval midgut in response to metal exposure, and in the adult female midgut in response to iron or cadmium exposure, or a blood meal. The cDNA encodes a protein, designated Aedes aegypti intestinal mucin 1 (AEIMUC1), which has similarities with invertebrate intestinal mucins and peritrophins, and vertebrate mucins. Proline, serine and threonine comprise 30% of the amino acid composition of AEIMUC1, a characteristic of mucins. AEIMUC1 contains three cysteine-rich domains, two of which flank a proline/serine/threonine-rich domain, a feature shared by many mucin genes. This is the first report on the isolation of a metal-responsive gene from an aquatic insect.

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页码：419 / 426

页数：8

相关论文

共 32 条

[1] The MUC2 gene product: a human intestinal mucin [J].

Allen, A ;

Hutton, DA ;

Pearson, JP .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 1998, 30 (07) :797-801

[2] GENETIC AND MOLECULAR ECOTOXICOLOGY - A RESEARCH FRAMEWORK [J].

ANDERSON, S ;

SADINSKI, W ;

SHUGART, L ;

BRUSSARD, P ;

DEPLEDGE, M ;

FORD, T ;

HOSE, J ;

STEGEMAN, J ;

SUK, W ;

WIRGIN, I ;

WOGAN, G .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1994, 102 :3-8

[3] Bactericidal activity and poly-L-proline II conformation of the tandem repeat sequence of human salivary mucin glycoprotein (MG2) [J].

Antonyraj, KJ ;

Karunakaran, T ;

Raj, PA .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1998, 356 (02) :197-206

[4] Molecular and genetic ecotoxicologic approaches to aquatic environmental bioreporting [J].

Beaty, BJ ;

Black, WC ;

Carlson, JO ;

Clements, WH ;

DuTeau, N ;

Harrahy, E ;

Nuckols, J ;

Olson, KE ;

Rayms-Keller, A .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1998, 106 :1395-1407

[5] THE ARTHROPOD INITIATOR - THE CAPSITE CONSENSUS PLAYS AN IMPORTANT ROLE IN TRANSCRIPTION [J].

CHERBAS, L ;

CHERBAS, P .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1993, 23 (01) :81-90

[6] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[7] INTEGRATED LABORATORY AND FIELD APPROACH FOR ASSESSING IMPACTS OF HEAVY-METALS AT THE ARKANSAS RIVER, COLORADO [J].

CLEMENTS, WH ;

KIFFNEY, PM .

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 1994, 13 (03) :397-404

[8] INVERTEBRATE ORGANISMS AS BIOLOGICAL INDICATORS OF HEAVY-METAL POLLUTION [J].

DALLINGER, R .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1994, 48 (01) :27-31

[9]

GOLDERMAN L, 1985, ISRAEL J MED SCI, V21, P410

[10] AQUATIC INSECTS AND TRACE-METALS - BIOAVAILABILITY, BIOACCUMULATION, AND TOXICITY [J].

HARE, L .

CRITICAL REVIEWS IN TOXICOLOGY, 1992, 22 (5-6) :327-369

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