Separation of tripalmitin from its hydrolysis products by simple isocratic reversed-phase high-performance liquid chromatography

An isocratic reversed-phase high-performance liquid-chromatographic method was developed for monitoring the lipase-catalyzed hydrolysis of solid triglycerides in supercritical carbon dioxide. The reaction products obtained consist of free fatty acids, monoglycerides, diglycerides, and unreacted triglycerides. For the method, developed with tripalmitin, a mobile phase of acetonitrile/chloroform/acetic acid (50:50:1, vol/vol/vol), a C-18 column, and refractive index detection were used. Analysis time is 7 min. Response factors were determined for each neutral lipid class, which permitted quantitation of the hydrolysis product mixture.