Flow cytometric analysis of microorganisms

被引:52
作者
Winson, MK [1 ]
Davey, HM [1 ]
机构
[1] Univ Wales, Inst Biol Sci, Aberystwyth SY23 3DD, Dyfed, Wales
来源
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY | 2000年 / 21卷 / 03期
关键词
D O I
10.1006/meth.2000.1003
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The application of flow cytometry to microorganisms is as old as the technique itself, but it has historically been underexploited for microbial applications. This is now being reversed and microbiologists are ideally placed to benefit from recent technological advances. White earlier papers demonstrated the use of flow cytometry far studies of viability and taxonomy, recent developments in bioinformatics and reporter gene technologies are leading to novel applications in microbiology. Variants of green fluorescent protein have been used for the study of conditional microbial gene regulation in medically important host-pathogen interactions and fluorescence-activated cell sorting is being applied to the isolation of novel mutants in directed evolution studies. This paper reviews the reasons for the delay in the application of Row cytometry to microbial problems, the range of applications, and their limitations and considers the progress made in developing new strategies for use in microbiological investigations. (C) 2000 Academic Press.
引用
收藏
页码:231 / 240
页数:10
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