Effects of α-tocopherol and mixed tocopherol supplementation on markers of oxidative stress and inflammation in type 2 diabetes

被引:88
作者
Wu, Jason H. Y.
Ward, Natalie C.
Indrawan, Adeline P.
Almeida, Coral-Ann
Hodgson, Jonathan M.
Proudfoot, Julie M.
Puddey, Ian B.
Croft, Kevin D.
机构
[1] Univ Western Australia, Sch Med & Pharmacol, Perth, WA 6847, Australia
[2] Royal Perth Hosp & Murdoch Univ, Ctr Clin Immunol & Biomed Stat, Perth, WA, Australia
关键词
D O I
10.1373/clinchem.2006.076992
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Vitamin E isomers may protect against atherosclerosis. The aim of this study was to compare the effects of supplementation with either alpha-tocopherol (alpha T) or mixed tocopherols rich in gamma-tocopherol (gamma T) on markers of oxidative stress and inflammation in patients with type 2 diabetes. Methods: In a double-blind, placebo-controlled trial, 55 patients with type 2 diabetes were randomly assigned to receive (500 mg/day) (a) alpha T, (b) mixed tocopherols, or (0 placebo for 6 weeks. Cellular tocopherols, plasma and urine F-2-isoprostanes, erythrocyte antioxidant enzyme activities, plasma inflammatory markers, and ex vivo assessment of eicosanoid synthesis were analyzed pre- and postsupplementation. Results: Neutrophil alpha T and gamma T increased (both P < 0.001) with mixed tocopherol supplementation, whereas alpha T (P < 0.001) increased and gamma T decreased (P < 0.005) after alpha T supplementation. Both alpha T and mixed tocopherol supplementation resulted in reduced plasma F-2-isoprostanes (P < 0.001 and P = 0.001, respectively) but did not affect 24-h urinary F-2-isoprostanes or erythrocyte antioxidant enzyme activities. Neither aT nor mixed tocopherol supplementation affected plasma C-reactive protein, interleukin 6, tumor necrosis factor-alpha, or monocyte chemoattractant protein-1. Stimulated neutrophil leukotriene B-4 production decreased significantly in the mixed tocopherol group (P = 0.02) but not in the alpha T group (P = 0.15). Conclusions: The ability of tocopherols to reduce systemic oxidative stress suggests potential benefits of vitamin E supplementation in patients with type 2 diabetes. In populations with well-controlled type 2 diabetes, supplementation with either alpha T or mixed tocopherols rich in gamma T is unlikely to confer further benefits in reducing inflammation. (c) 2007 American Association for Clinical Chemistry.
引用
收藏
页码:511 / 519
页数:9
相关论文
共 40 条
[1]   Effect of statin therapy on C-reactive protein levels - The Pravastatin Inflammation/CRP Evaluation (PRINCE): A randomized trial and cohort study [J].
Albert, MA ;
Danielson, E ;
Rifai, N ;
Ridker, PM .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2001, 286 (01) :64-70
[2]   Vitamin E mediates cell signaling and regulation of gene expression [J].
Azzi, A ;
Gysin, R ;
Kempná, P ;
Munteanu, A ;
Negis, Y ;
Villacorta, L ;
Visarius, T ;
Zingg, JM .
VITAMIN E AND HEALTH, 2004, 1031 :86-95
[3]  
BOYUM A, 1968, SCAND J CLIN LAB INV, VS 21, P77
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   Long-term combined supplementations with α-tocopherol and vitamin C have no detectable anti-inflammatory effects in healthy men [J].
Bruunsgaard, H ;
Poulsen, HE ;
Pedersen, BK ;
Nyyssönen, K ;
Kaikkonen, J ;
Salonen, JT .
JOURNAL OF NUTRITION, 2003, 133 (04) :1170-1173
[6]   Association between 5-lipoxygenase expression and plaque instability in humans [J].
Cipollone, F ;
Mezzetti, A ;
Fazia, ML ;
Cuccurullo, C ;
Iezzi, A ;
Ucchino, S ;
Spigonardo, F ;
Bucci, M ;
Cuccurullo, F ;
Prescott, SM ;
Stafforini, DM .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2005, 25 (08) :1665-1670
[7]   Overexpression of functionally coupled cyclooxygenase-2 and prostaglandin E synthase in symptomatic atherosclerotic plaques as a basis of prostaglandin E2-dependent plaque instability [J].
Cipollone, F ;
Prontera, C ;
Pini, B ;
Marini, M ;
Fazia, M ;
De Cesare, D ;
Iezzi, A ;
Ucchino, S ;
Boccoli, G ;
Saba, V ;
Chiarelli, F ;
Cuccurullo, F ;
Mezzetti, A .
CIRCULATION, 2001, 104 (08) :921-927
[8]  
Clarke MW, 2006, AM J CLIN NUTR, V83, P95
[9]  
CROFT KD, 1991, EICOSANOIDS, V4, P75
[10]   Alpha tocopherol supplementation decreases serum C-reactive protein and monocyte interleukin-6 levels in normal volunteers and Type 2 diabetic patients [J].
Devaraj, S ;
Jialal, I .
FREE RADICAL BIOLOGY AND MEDICINE, 2000, 29 (08) :790-792