Preparation and application of magnetic poly(styrene-glycidyl methacrylate) microspheres

被引：32

作者：

Chung, Ting-Hao ^{[1
]}

Pan, Hsiao-Chun ^{[1
]}

Lee, Wen-Chien ^{[1
]}

机构：

[1] Natl Chung Cheng Univ, Dept Chem Engn, Chiayi 621, Taiwan

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2007年 / 311卷 / 01期

关键词：

magnetic microsphere; PS-GMA; polymer microsphere; DNA hybridization; superparamagnetic nanoparticle;

D O I：

10.1016/j.jmmm.2006.11.165

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A novel method for the preparation of polymer-based, magnetic microspheres is proposed. Pre-made, poly(styrene-glycidyl methacrylate) (PS-GMA) particles of micron size were swollen by a mixture of N-methyl-2-pyrrolidone and water, and then incubated with superparamagnetic nanoparticles. The nanoparticles were allowed to diffuse into polymer microspheres during the incubation, became entrapped and made the polymer microspheres superparamagnetic. These magnetic PS-GMA microspheres were chemically modified and then coupled with single-stranded oligonucleotides as probes for DNA hybridization. The immobilized probes showed repeatable capture of target oligonucleotides. (C) 2006 Elsevier B.V. All rights reserved.

引用

页码：36 / 40

页数：5