Synergistic inhibition of LDL oxidation by phytoestrogens and ascorbic acid

被引:81
作者
Hwang, J
Sevanian, A
Hodis, HN
Ursini, F
机构
[1] Univ So Calif, Sch Pharm, Dept Mol Pharmacol & Toxicol, Los Angeles, CA 90089 USA
[2] Div Cardiol, Atherosclerosis Res Unit, Los Angeles, CA USA
[3] Univ Padua, Dept Biol Chem, Padua, Italy
关键词
lipid peroxidation; antioxidant; copper; genistein; daidzein; equol; apoB-100; free radicals;
D O I
10.1016/S0891-5849(00)00322-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Increasing evidence indicates that oxidative modification of low-density lipoprotein (LDL) is an important determinant in atherogenesis, and following menopause, the incidence of coronary heart disease is as prevalent in women as it is in men. Estrogen has been demonstrated to inhibit the susceptibility of LDL to be oxidized, and more recently the use of phytoestrogens has been considered for estrogen replacement therapy. In this study the antioxidant activity of the three major phytoestrogens: genistein, daidzein, and equol were measured in terms of LDL oxidative susceptibility. Increasing levels of genistein, daidzein, and equol inhibited LDL oxidation, and this inhibitory effect was further enhanced in the presence of ascorbic acid. The synergism exhibited by these compounds is of clinical importance to phytoestrogen therapy since the efficacy of phytoestrogens as effective antioxidants is evident at concentration well within the range found in the plasma of subjects consuming soy products. However, this synergism, combined with the low reactivity of the phytoestrogens with peroxyl radicals, suggests that an antioxidant mechanism other then free radical scavenging reactions account for the phytoestrogen antioxidant effect, A structural basis for inhibition of LDL oxidation involving interaction of the phytoestrogens with apoB-100 is postulated. (C) 2000 Elsevier Science Inc.
引用
收藏
页码:79 / 89
页数:11
相关论文
共 39 条
[1]   Phyto-oestrogens and Western diseases [J].
Adlercreutz, H ;
Mazur, W .
ANNALS OF MEDICINE, 1997, 29 (02) :95-120
[2]  
AKIYAMA T, 1987, J BIOL CHEM, V262, P5592
[3]  
[Anonymous], 1975, JAMA-J AM MED ASSOC, V231, P360
[4]   A SPECTROPHOTOMETRIC MICROTITER-BASED ASSAY FOR THE DETECTION OF HYDROPEROXY DERIVATIVES OF LINOLEIC-ACID [J].
AUERBACH, BJ ;
KIELY, JS ;
CORNICELLI, JA .
ANALYTICAL BIOCHEMISTRY, 1992, 201 (02) :375-380
[5]  
Barnes S, 1996, ADV EXP MED BIOL, V401, P87
[6]  
BERENBAUM MC, 1989, PHARMACOL REV, V41, P93
[7]   The role of oxidized lipoproteins in atherogenesis [J].
Berliner, JA ;
Heinecke, JW .
FREE RADICAL BIOLOGY AND MEDICINE, 1996, 20 (05) :707-727
[8]  
BORS W, 1990, METHOD ENZYMOL, V186, P343
[9]   SIMPLE DETERMINATION OF RETINOL, ALPHA-TOCOPHEROL AND CAROTENOIDS (LUTEIN, ALL-TRANS-LYCOPENE, ALPHA-CAROTENE AND BETA-CAROTENE) IN HUMAN PLASMA BY ISOCRATIC LIQUID-CHROMATOGRAPHY [J].
BUI, MH .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1994, 654 (01) :129-133
[10]  
BURR ML, 1974, LANCET, V1, P163