A decontamination and sterilization protocol employed during reuse of cardiac electrophysiology catheters inactivates human immunodeficiency virus

被引:8
作者
Druce, JD
Russell, JS
Birch, CJ
Yates, LA
Harper, RW
Smolich, JJ
机构
[1] Monash Med Ctr, Ctr Heart & Chest Res, Cardiol Unit, Clayton, Vic 3168, Australia
[2] Victorian Infect Dis Reference Lab, Melbourne, Australia
[3] Monash Univ, Dept Med, Ctr Heart & Chest Res, Clayton, Vic 3168, Australia
关键词
D O I
10.1086/502190
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
OBJECTIVE: To assess the effect of a standard decontamination and sterilization protocol employed during reuse of cardiac electrophysiology (EP) catheters on human immunodeficiency virus (HIV). SETTING: Public health viral research laboratory. METHODS: Studies were performed on distal, electrode-containing segments of 40 EP catheters previously used in up to 10 clinical EP procedures. EP catheter segments were immersed for 1 hour in blood contaminated with a high titer of HIV. After air drying for 2 hours, subgroups of 8 EP catheters were subjected to either (1) no treatment, (2) washing in general purpose detergent, (3) washing in enzyme cleaner, (4) sterilization in ethylene oxide, or (5) the full protocol of sequential detergent-enzyme cleaner-ethylene oxide exposure. HIV infectivity after treatment was determined by measuring HIV RNA and, in cell culture studies, assessing HIV-induced cytopathic effects (CPEs) and supernatant HIV-specific p24 antigen content. RESULTS: With no treatment, all catheters had high HIV RNA levels associated with CPEs and high p24 antigen levels. After washing in detergent, 5 of 8 catheters had HIV RNA detected, but without CPEs or p24 antigen. HIV RNA was detected in all catheters after washing in enzyme cleaner, with CPEs and a high p24 antigen level in 1 of 8 catheters. HIV RNA, CPEs, and p24 antigen were absent after ethylene oxide. After the full protocol, HIV RNA levels were undetectable (n = 7) or low (n = 1), without evidence of CPEs or p24 antigen. CONCLUSION: Appropriate decontamination and sterilization of EP catheters during reuse is highly effective in inactivating HIV.
引用
收藏
页码:184 / 190
页数:7
相关论文
共 20 条
[1]   REPEATED USE OF ABLATION CATHETERS - A PROSPECTIVE-STUDY [J].
AVITALL, B ;
KHAN, M ;
KRUM, D ;
JAZAYERI, M ;
HARE, J .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1993, 22 (05) :1367-1372
[2]   The expanding role of the cardiologist in the care of HIV infected patients [J].
Barbaro, G ;
Fisher, SD ;
Pellicelli, AM ;
Lipshultz, SE .
HEART, 2001, 86 (04) :365-367
[3]  
Barbaro G, 1996, Cardiologia, V41, P1199
[4]   The safety of reusing ablation catheters with temperature control and the need for a validation protocol and guidelines for reprocessing [J].
Blomström-Lundqvist, C .
PACE-PACING AND CLINICAL ELECTROPHYSIOLOGY, 1998, 21 (12) :2563-2570
[5]   TEMPERATURE MONITORING DURING RADIOFREQUENCY CATHETER ABLATION PROCEDURES USING CLOSED-LOOP CONTROL [J].
CALKINS, H ;
PRYSTOWSKY, E ;
CARLSON, M ;
KLEIN, LS ;
SAUL, JP ;
KAY, GN ;
DAILEY, S ;
EPSTEIN, A ;
PLUMB, V ;
WHARTON, M ;
KANTER, R ;
SORRENTINO, R ;
GREENFIELD, RA ;
WALSH, E ;
WALDO, A ;
BIBLO, L ;
JOHNSON, N ;
LIEBERMAN, R ;
ROSENBAUM, D ;
HUANG, SKS ;
MITTLEMAN, RS ;
WAGSHAL, AB ;
GREENE, T ;
LIEM, B ;
LAUER, M ;
SUNG, R ;
YOUNG, C ;
EVANS, J ;
CASE, C ;
HACKETT, K ;
MILES, W ;
ZIPES, D ;
LAWRENCE, J ;
TOMASELLI, G ;
BERGER, R .
CIRCULATION, 1994, 90 (03) :1279-1286
[6]   PATIENT-TO-PATIENT TRANSMISSION OF HIV IN PRIVATE SURGICAL CONSULTING ROOMS [J].
CHANT, K ;
LOWE, D ;
RUBIN, G ;
MANNING, W ;
ODONOUGHUE, R ;
LYLE, D ;
LEVY, M ;
MOREY, S ;
KALDOR, J ;
GARSIA, R ;
PENNY, R ;
MARRIOTT, D ;
CUNNINGHAM, A ;
TRACY, GD .
LANCET, 1993, 342 (8886-7) :1548-1549
[7]   THE 1990 FLORIDA DENTAL INVESTIGATION - THE PRESS AND THE SCIENCE [J].
CIESIELSKI, CA ;
MARIANOS, DW ;
SCHOCHETMAN, G ;
WITTE, JJ ;
JAFFE, HW .
ANNALS OF INTERNAL MEDICINE, 1994, 121 (11) :886-888
[8]  
Druce J, 2001, DISINFECTION STERILI, P573
[9]   SUSCEPTIBILITY OF HIV TO INACTIVATION BY DISINFECTANTS AND ULTRAVIOLET-LIGHT [J].
DRUCE, JD ;
JARDINE, D ;
LOCARNINI, SA ;
BIRCH, CJ .
JOURNAL OF HOSPITAL INFECTION, 1995, 30 (03) :167-180
[10]   SUCCESS OF REUSE OF CARDIAC ELECTRODE CATHETERS [J].
DUNNIGAN, A ;
ROBERTS, C ;
MCNAMARA, M ;
BENSON, DW ;
BENDITT, DG .
AMERICAN JOURNAL OF CARDIOLOGY, 1987, 60 (10) :807-810