A type III-like restriction endonuclease functions as a major barrier to horizontal gene transfer in clinical Staphylococcus aureus strains

被引:90
作者
Corvaglia, Anna R. [1 ]
Francois, Patrice [2 ]
Hernandez, David [2 ]
Perron, Karl [1 ]
Linder, Patrick [1 ]
Schrenzel, Jacques [2 ]
机构
[1] Univ Geneva, Univ Med Ctr, Dept Microbiol & Mol Med, CH-1211 Geneva 4, Switzerland
[2] Univ Hosp Geneva, Dept Internal Med, Genom Res Lab, CH-1211 Geneva 14, Switzerland
基金
瑞士国家科学基金会;
关键词
antibiotic resistance; transformation; targetron; MODIFICATION SYSTEM; ESCHERICHIA-COLI; DNA; ENZYMES; PLASMID-P15B; RESISTANCE; VERSATILE; PHAGE-P1; CLEAVAGE; AGR;
D O I
10.1073/pnas.1000489107
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Staphylococcus aureus is an versatile pathogen that can cause life-threatening infections. Depending on the clinical setting, up to 50% of S. aureus infections are caused by methicillin-resistant strains (MRSA) that in most cases are resistant to many other antibiotics, making treatment difficult. The emergence of community-acquired MRSA drastically changed the picture by increasing the risk of MRSA infections. Horizontal transfer of genes encoding for antibiotic resistance or virulence factors is a major concern of multidrug-resistant S. aureus infections and epidemiology. We identified and characterized a type III-like restriction system present in clinical S. aureus strains that prevents transformation with DNA from other bacterial species. Interestingly, our analysis revealed that some clinical MRSA strains are deficient in this restriction system, and thus are hypersusceptible to the horizontal transfer of DNA from other species, such as Escherichia coli, and could easily acquire a vancomycin-resistance gene from enterococci. Inactivation of this restriction system dramatically increases the transformation efficiency of clinical S. aureus strains, opening the field of molecular genetic manipulation of these strains using DNA of exogenous origin.
引用
收藏
页码:11954 / 11958
页数:5
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