Programmed DNA deletion as an RNA-guided system of genome defense

被引：153

作者：

Yao, MC ^{[1
]}

Fuller, P ^{[1
]}

Xi, XH ^{[1
]}

机构：

[1] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA

来源：

SCIENCE | 2003年 / 300卷 / 5625期

关键词：

D O I：

10.1126/science.1084737

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genomewide DNA rearrangements occur in many eukaryotes during development, but their functions and mechanisms are poorly understood. Previous studies have implicated a sequence-recognition mechanism based on RNA-mediated interactions between nuclei in ciliated protozoa. In this study, we found that the process recognized and deleted a foreign gene integrated in a Tetrahymena chromosome, suggesting an unusual mechanism of genome surveillance. We further found that injection of double-stranded RNA into the cell at specific developmental stages triggers efficient deletion of the targeted genomic regions. Together the results indicate an RNA-based mechanism that directs genomewide DNA rearrangements and serves to disable invading genetic agents.

引用

页码：1581 / 1584

页数：4

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