Dynamic proteomic overview of glioblastoma cells (A172) exposed to perillyl alcohol

被引：20

作者：

da Gama Fischer, Juliana de Saldanha ^{[1
,2
,3
]}

Liao, Lujian ^{[1
]}

Carvalho, Paulo C. ^{[1
,4
]}

Barbosa, Valmir C. ^{[4
]}

Domont, Gilberto B. ^{[2
,3
]}

da Costa Carvalho, Maria da Gloria ^{[5
]}

Yates, John R., III ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA

[2] Univ Fed Rio de Janeiro, Prot Chem Lab, Inst Chem, Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Rio de Janeiro Prote Network, Rio De Janeiro, Brazil

[4] Univ Fed Rio de Janeiro, COPPE, Syst Engn & Comp Sci Program, BR-21945 Rio De Janeiro, Brazil

[5] Univ Fed Rio de Janeiro, Carlos Chagas Filho Biophys Inst, Lab Control Gene Express, BR-21941 Rio De Janeiro, Brazil

来源：

JOURNAL OF PROTEOMICS | 2010年 / 73卷 / 05期

基金：

美国国家卫生研究院;

关键词：

Perillyl alcohol; Glioblastoma; Time-course; A172; cells; MudPIT; GSK3; beta; ERK; SHOTGUN; TOOL;

D O I：

10.1016/j.jprot.2010.01.003

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Perillyl alcohol (POH) is a naturally occurring terpene and a promising chemotherapeutic agent for glioblastoma multiform, yet, little is known about its molecular effects Here we present results of a semi-quantitative proteomic analysis of A172 cells exposed to POH for different time-periods (1', 10', 30', 60', 4 h, and 24 h) The analysis identified more than 4000 proteins, which were clustered using PatternLab for proteomics and then linked to Ras signaling, tissue homeostasis, induction of apoptosis, metallopeptidase activity, and ubiquitin-protein ligase activity Our results make available one of the most complete protein repositories for the A172 Moreover, we detected the phosphorylation of GSK3 beta (Glycogen synthase kinase) and the inhibition of ERK's (extracellular signal regulated kinase) phosphorylation after 10', which suggests a new mechanism of POH's activation for apoptosis (C) 2010 Elsevier B V All rights reserved

引用

页码：1018 / 1027

页数：10

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