Gsα meets its target -: shedding light on a key signal transduction event

被引：5

作者：

Dumas, JJ ^{[1
]}

Lambright, DG ^{[1
]}

机构：

[1] Univ Massachusetts, Med Ctr, Program Mol Med, Worcester, MA 01605 USA

来源：

STRUCTURE WITH FOLDING & DESIGN | 1998年 / 6卷 / 04期

关键词：

D O I：

10.1016/S0969-2126(98)00042-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recently determined crystal structure of G(s alpha) bound to a catalytically active form of adenylyl cyclase reveals the location of the enzyme's active site and provides the first view of heterotrimeric G protein alpha subunit activating a downstream effector, Comparison with the structure of a catalytically inactive form of adenylyl cyclase suggests a plausible allosteric mechanism whereby the synergistic activators G(s alpha) and forskolin stimulate the activity of adenylyl cyclase, (C) Current Biology Ltd ISSN 0969-2126.

引用

页码：407 / 411

页数：5

共 18 条

[1] IDENTIFICATION OF EFFECTOR-ACTIVATING RESIDUES OF GS-ALPHA [J].

BERLOT, CH ;

BOURNE, HR .

CELL, 1992, 68 (05) :911-922

[2] Expression and purification of the Saccharomyces cerevisiae alpha-factor receptor (Ste2p), a 7-transmembrane-segment G protein-coupled receptor [J].

David, NE ;

Gee, M ;

Andersen, B ;

Naider, F ;

Thorner, J ;

Stevens, RC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (24) :15553-15561

[3] Interactions of forskolin and ATP with the cytosolic domains of mammalian adenylyl cyclase [J].

Dessauer, CW ;

Scully, TT ;

Gilman, AG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (35) :22272-22277

[4] Purification and characterization of a soluble form of mammalian adenylyl cyclase [J].

Dessauer, CW ;

Gilman, AG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (28) :16967-16974

[5] MOLECULAR AND FUNCTIONAL DIVERSITY OF MAMMALIAN G(S)-STIMULATED ADENYLYL CYCLASES [J].

IYENGAR, R .

FASEB JOURNAL, 1993, 7 (09) :768-775

[6] X-ray structure of bacteriorhodopsin at 2.5 angstroms from microcrystals grown in lipidic cubic phases [J].

PebayPeyroula, E ;

Rummel, G ;

Rosenbusch, JP ;

Landau, EM .

SCIENCE, 1997, 277 (5332) :1676-1681

[7] Structure at 1.65 angstrom of RhoA and its GTPase-activating protein in complex with a transition-state analogue [J].

Rittinger, K ;

Walker, PA ;

Eccleston, JF ;

Smerdon, SJ ;

Gamblin, SJ .

NATURE, 1997, 389 (6652) :758-762

[8] The Ras-RasGAP complex: Structural basis for GTPase activation and its loss in oncogenic Ras mutants [J].

Scheffzek, K ;

Ahmadian, MR ;

Kabsch, W ;

Wiesmuller, L ;

Lautwein, A ;

Schmitz, F ;

Wittinghofer, A .

SCIENCE, 1997, 277 (5324) :333-338

[9] GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL-STRUCTURE OF TRANSDUCIN ALPHA-CENTER-DOT-GDP-CENTER-DOT-ALF4(-) [J].

SONDEK, J ;

LAMBRIGHT, DG ;

NOEL, JP ;

HAMM, HE ;

SIGLER, PB .

NATURE, 1994, 372 (6503) :276-279

[10] Crystal structure of the adenylyl cyclase activator G(S alpha) [J].

Sunahara, RK ;

Tesmer, JJG ;

Gilman, AG ;

Sprang, SR .

SCIENCE, 1997, 278 (5345) :1943-1947

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