Measurement of molecular association in drug:cyclodextrin inclusion complexes with improved H-1 NMR studies

The molecular association of haloperidol with hydroxypropyl-B-cyclodextrin, expressed by the binding constant of the inclusion complex formed, was calculated from the changes on the H-1 NMR spectra of the drug in the presence of the cyclodextrin. The stoichiometry of the complex was calculated by use of the continuous variation method (Job plot), and found to be 1:1. The binding constant for the 1:1 complex was calculated using improved non-linear models, which were solved by non-linear least-squares regression analysis, applying an iteration procedure. Three improved mathematical models for more accurate calculation of binding constants are proposed. The models are free from any assumptions and from practical or theoretical shortcomings.