An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress

被引:250
作者
Shen, YG
Zhang, WK
He, SJ
Zhang, JS
Liu, Q
Chen, SY [1 ]
机构
[1] Chinese Acad Sci, Genet Inst, Plant Biotechnol Lab, Beijing 100101, Peoples R China
[2] Tsinghua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
关键词
DRE-binding protein; one-hybrid; abiotic stresses; Triticum aestivum L;
D O I
10.1007/s00122-002-1131-x
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
We characterize one transcription factor of DRE-binding proteins (TaDREB1) that was isolated from a drought-induced cDNA library of wheat (Triticum aestivum L.). TaDREB1 contains one conserved EREBP/AP2 domain, and shows similarity with Arabidopsis thaliana DREB family members in both overall amino-acid sequences and the secondary structure arrangement within the DNA-binding motifs. In yeast one-hybrid system, TaDREB1, can specially activate the genes fused with the promoter containing three tandemly repeated copies of the wild-type DRE sequence: TACCGACAT. In different wheat cultivars, the Ta DREB1 gene is induced by low temperature, salinity and drought; and the expression of Wcs120 that contains DRE motifs in its promoter is closely related to the expression of TaDRE1. These results suggest that TaDREB1 functions as a DRE-binding transcription factor in wheat. We also observed the dwarf phenotype in transgenic rice (TO) overexpressing TaDREB1.
引用
收藏
页码:923 / 930
页数:8
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