Highly resolved chemical imaging of living cells by using synchrotron infrared microspectrometry

被引：228

作者：

Jamin, N

Dumas, P

Moncuit, J

Fridman, WH

Teillaud, JL

Carr, GL

Williams, GP

机构：

[1] Ctr Univ Paris Sud, Utilisat Rayonnement Electromagnet Lab, F-91405 Orsay, France

[2] CEA, Inst Natl Sci & Tech Nucl, F-91191 Gif Sur Yvette, France

[3] Ctr Univ Paris Sud, CNRS, Lab Spect Infrarouge & Raman, F-91405 Orsay, France

[4] Inst Curie, INSERM, U255, F-75248 Paris 05, France

[5] Brookhaven Natl Lab, Natl Synchrotron Light Source, Upton, NY 11974 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 09期

关键词：

D O I：

10.1073/pnas.95.9.4837

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Using synchrotron radiation as an ultrabright infrared source, we have been able to map the distributions of functional groups such as proteins, lipids, and nucleic acids inside a single living cell with a spatial resolution of a few microns. In particular, we have mapped the changes in the lipid and protein distributions in both the final stages of cell division and also during necrosis.

引用

页码：4837 / 4840

页数：4

共 14 条

[1] REGULATORY EFFECTS OF IGG-BF ON HYBRIDOMA-B CELLS - MOLECULAR CHARACTERIZATION OF VARIANT CELL-LINES [J].